RNF20 Rabbit Monoclonal Antibody (CAB4784)

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CAB4784
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Description

Technical Manual

RNF20 Rabbit Monoclonal Antibody

Product Name:RNF20 Rabbit Monoclonal Antibody
Product SKU:CAB4784
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012]

Immunogen:A synthesized peptide derived from human RNF20
Sequence:

Email for sequence

Gene ID:56254
Swiss Prot:Q5VTR2
Synonyms:BRE1; BRE1A; hBRE1
Calculated MW:114kDa
Observed MW:120KDa
Reactivity:Human, Mouse
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting 
Positive Samples:HeLa, 293T, C2C12, Mouse testis
Cellular Location:

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RNF20 Rabbit Monoclonal Antibody (CAB4784)

Western blot - RNF20 Rabbit mAb (CAB4784)

Uniprot Information

UniProt Protein Function:RNF20: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with p53/TP53 and WAC. Interacts with PAF1; the interaction mediates the association of the PAF1 and RNF20/40 complexes which is a prerequsite for recruitment of UBE2A/B. Belongs to the BRE1 family.
UniProt Protein Details:

Protein type:EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: nucleoplasm; nucleolus; nucleus; ubiquitin ligase complex

Molecular Function:protein binding; mRNA 3'-UTR binding; histone binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; chromatin binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; histone monoubiquitination; protein polyubiquitination; positive regulation of histone methylation; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of mitotic cell cycle; histone H2B ubiquitination; negative regulation of cell migration

NCBI Summary:The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012]
UniProt Code:Q5VTR2
NCBI GenInfo Identifier:84027766
NCBI Gene ID:56254
NCBI Accession:Q5VTR2.2
UniProt Secondary Accession:Q5VTR2,Q2TB34, Q69YL5, Q6P527, Q8N3J4, Q96JD3, Q9H9Y7 Q9HA51, Q9NUR4, Q9NWQ3, Q9NX83, A7MCT5,
UniProt Related Accession:Q5VTR2
Molecular Weight:975
NCBI Full Name:E3 ubiquitin-protein ligase BRE1A
NCBI Synonym Full Names:ring finger protein 20, E3 ubiquitin protein ligase
NCBI Official Symbol:RNF20 
NCBI Official Synonym Symbols:BRE1; BRE1A; hBRE1 
NCBI Protein Information:E3 ubiquitin-protein ligase BRE1A; BRE1-A; homolog of S. cerevisiae BRE1; BRE1 E3 ubiquitin ligase homolog
UniProt Protein Name:E3 ubiquitin-protein ligase BRE1A
UniProt Synonym Protein Names:RING finger protein 20
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:RNF20 
UniProt Entry Name:BRE1A_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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