Anti-S100A8 Antibody (CAB20474)

Product Name:	S100A8 Rabbit pAb
Product Code:	CAB20474
Size:	50uL, 100uL
Synonyms:	p, Ca, MR, p8, B8A, CFA, B8Ag, CFAg, CP-1, Caga, MRP8, 60B8A, CP-10, 60B8Ag, AI323541
Applications:	WB, IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of Mouse S100A8.

Applications:	WB, IHC
Recommended Dilutions:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse lung, Mouse spleen, Rat spleen

Immunogen:	Recombinant protein of Mouse S100A8.
Purification Method:	Affinity purification
Storage:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	20201
Uniprot:	P27005
Calculated MW:	10kDa
Observed MW:	11KDa

UniProt Protein Function:	S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 3 F1\|3 39.9 cM Cellular Component: extracellular space; nucleus Biological Process: astrocyte development; autophagy; caspase activation; leukocyte migration during inflammatory response; neutrophil chemotaxis; peptidyl-cysteine S-nitrosylation; positive regulation of inflammatory response; positive regulation of peptide secretion
UniProt Code:	P27005
NCBI GenInfo Identifier:	1173338
NCBI Gene ID:	20201
NCBI Accession:	P27005.3
UniProt Secondary Accession:	P27005,P31724,
UniProt Related Accession:	P27005
Molecular Weight:	10,295 Da
NCBI Full Name:	Protein S100-A8
NCBI Synonym Full Names:	S100 calcium binding protein A8 (calgranulin A)
NCBI Official Symbol:	S100a8
NCBI Official Synonym Symbols:	p8; B8Ag; CFAg; Caga; MRP8; CP-10; 60B8Ag; AI323541
NCBI Protein Information:	protein S100-A8
UniProt Protein Name:	Protein S100-A8
UniProt Synonym Protein Names:	Calgranulin-A; Chemotactic cytokine CP-10; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; Pro-inflammatory S100 cytokine; S100 calcium-binding protein A8
Protein Family:	Protein
UniProt Gene Name:	S100a8