Anti-SARNP Antibody (CAB13701)

Antibody Name:	Anti-SARNP Antibody
Antibody SKU:	CAB13701
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human SARNP (NP_149073.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	293T, HepG2, BxPC-3, U-87MG, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human SARNP (NP_149073.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATE TVEL HKLK LAEL KQEC LARG LETK GIKQ DLIH RLQA YLEE HAEE EANE EDVL GDET EEEE TKPI ELPV KEEE PPEK TVDV AAEK KVVK ITSE IPQT ERMQ KRAE RFNV PVSL ESKK AARA ARFG ISSV PTKG LSSD NKPM VNLD KLKE RAQR FGLN VSSI SRKS EDDE KLKK RKER FGIV TSSA GTGT TEDT EAKK RKRA ERFG IA
Gene ID:	84324
Uniprot:	P82979
Cellular Location:	Nucleus, Nucleus speckle
Calculated MW:	23kDa
Observed MW:	29kDa

Synonyms:

SARNP, CIP29, HCC1, HSPC316, THO1

Background:

This gene encodes a protein that is upregulated in response to various cytokines. The encoded protein may play a role in cell cycle progression. A translocation between this gene and the myeloid/lymphoid leukemia gene, resulting in expression of a chimeric protein, has been associated with acute myelomonocytic leukemia. Pseudogenes exist on chromosomes 7 and 8. Alternatively spliced transcript variants have been described.

UniProt Protein Function:	CIP29: Binds both single-stranded and double-stranded DNA with higher affinity for the single-stranded form. Specifically binds to scaffold/matrix attachment region DNA. Also binds single- stranded RNA. Enhances RNA unwinding activity of DDX39A. May participate in important transcriptional or translational control of cell growth, metabolism and carcinogenesis.
UniProt Protein Details:	Protein type:RNA-binding Chromosomal Location of Human Ortholog: 12q13.2 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nuclear speck; nucleus Molecular Function:DNA binding Biological Process: regulation of translation; mRNA export from nucleus; transcription, DNA-dependent; regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a protein that is upregulated in response to various cytokines. The encoded protein may play a role in cell cycle progression. A translocation between this gene and the myeloid/lymphoid leukemia gene, resulting in expression of a chimeric protein, has been associated with acute myelomonocytic leukemia. Pseudogenes exist on chromosomes 7 and 8. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]
UniProt Code:	P82979
NCBI GenInfo Identifier:	18202440
NCBI Gene ID:	84324
NCBI Accession:	P82979.3
UniProt Secondary Accession:	P82979,Q9P066, A8K393,
UniProt Related Accession:	P82979
Molecular Weight:	210
NCBI Full Name:	SAP domain-containing ribonucleoprotein
NCBI Synonym Full Names:	SAP domain containing ribonucleoprotein
NCBI Official Symbol:	SARNP
NCBI Official Synonym Symbols:	HCC1; THO1; CIP29; HSPC316
NCBI Protein Information:	SAP domain-containing ribonucleoprotein; nuclear protein Hcc-1; hepatocellular carcinoma 1; cytokine induced protein 29 kDa; cytokine-induced protein of 29 kDa; proliferation associated cytokine-inducible protein CIP29; proliferation-associated cytokine-inducible protein CIP29
UniProt Protein Name:	SAP domain-containing ribonucleoprotein
UniProt Synonym Protein Names:	Cytokine-induced protein of 29 kDa; Nuclear protein Hcc-1; Proliferation-associated cytokine-inducible protein CIP29
Protein Family:	SAP domain-containing ribonucleoprotein
UniProt Gene Name:	SARNP
UniProt Entry Name:	SARNP_HUMAN

	Western blot analysis of extracts of various cell lines, using SARNP antibody (CAB13701) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Human colon using SARNP Rabbit pAb (CAB13701) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using SARNP antibody (CAB13701) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

SARNP Antibody (PACO05510)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)