Anti-Securin Antibody (CAB8307)

Antibody Name:	PTTG1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8307
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-202 of human PTTG1 (NP_001269311.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, Jurkat, HeLa, Mouse stomach, Mouse thymus, Mouse spleen, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-202 of human PTTG1 (NP_001269311.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATL IYVD KENG EPGT RVVA KDGL KLGS GPSI KALD GRSQ VSTP RFGK TFDA PPAL PKAT RKAL GTVN RATE KSVK TKGP LKQK QPSF SAKK MTEK TVKA KSSV PASD DAYP EIEK FFPF NPLD FESF DLPE EHQI AHLP LSGV PLMI LDEE RELE KLFQ LGPP SPVK MPSP PWES NLLQ SPSS ILST LDVE LPPV CCDI DI
Gene ID:	9232
Uniprot:	O95997
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	22kDa
Observed MW:	24kDa

Synonyms:

PTTG1, EAP1, HPTTG, PTTG, TUTR1, securin

Background:

The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	Securin: Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation. Belongs to the securin family.
UniProt Protein Details:	Protein type:Oncoprotein; Cell cycle regulation Chromosomal Location of Human Ortholog: 5q35.1 Cellular Component: cytoplasm; cytosol Molecular Function:protease inhibitor activity; protein binding; transcription factor activity Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; homologous chromosome segregation; protein ubiquitination during ubiquitin-dependent protein catabolic process; spermatogenesis; transcription from RNA polymerase II promoter
NCBI Summary:	The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
UniProt Code:	O95997
NCBI GenInfo Identifier:	29336594
NCBI Gene ID:	9232
NCBI Accession:	O95997.1
UniProt Related Accession:	O95997
Molecular Weight:
NCBI Full Name:	Securin
NCBI Synonym Full Names:	pituitary tumor-transforming 1
NCBI Official Symbol:	PTTG1
NCBI Official Synonym Symbols:	EAP1; PTTG; HPTTG; TUTR1
NCBI Protein Information:	securin
UniProt Protein Name:	Securin
UniProt Synonym Protein Names:	Esp1-associated protein; Pituitary tumor-transforming gene 1 protein; Tumor-transforming protein 1; hPTTG
UniProt Gene Name:	PTTG1
UniProt Entry Name:	PTTG1_HUMAN

	Western blot analysis of extracts of various cell lines, using PTTG1 antibody (CAB8307) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human lung cancer using PTTG1 antibody (CAB8307) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver using PTTG1 antibody (CAB8307) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using PTTG1 antibody (CAB8307) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
PTTG1 Antibody (PACO01377)	PTTG1 Colorimetric Cell-Based ELISA
PTTG1 Antibody (PACO11606)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)