Western blot analysis of various lysates, using SETD6 antibody (CAB22262) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The SETD6 Monoclonal Antibody (CAB22262) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through monoclonal technology, exhibits high specificity and sensitivity towards SETD6 protein in human samples, making it ideal for use in various research applications such as immunoprecipitation and immunofluorescence.SETD6 is known for its role in histone methylation and chromatin remodeling, ultimately impacting gene transcription and cell fate.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SETD6 Monoclonal Antibody
SKU:
CAB22262
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC55206
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SETD6
Positive Sample:
293F, MCF7, Mouse stomach, Rat liver
Cellular Localization:
Nucleus.
Calculated MW:
53kDa
Observed MW:
53kDa
This gene encodes a methyltransferase that adds a methyl group to the histone H2AZ, which is involved in nuclear receptor-dependent transcription. The protein also interacts with several endogenous proteins which are involved in nuclear hormone receptor signaling. A related pseudogene is located on chromosome 2. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
79918
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using SETD6 Rabbit mAb (CAB22262) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of various lysates, using SETD6 Rabbit mAb (CAB22262) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
