Anti-Smad5 Antibody (CAB0635)

Antibody Name:	Anti-Smad5 Antibody
Antibody SKU:	CAB0635
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 140-240 of human Smad5 (NP_005894.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat lung

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 140-240 of human Smad5 (NP_005894.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LVPR HNEF NPQH SLLV QFRN LSHN EPHM PQNA TFPD SFHQ PNNT PFPL SPNS PYPP SPAS STYP NSPA SSGP GSPF QLPA DTPP PAYM PPDD QMGQ DNSQ P
Gene ID:	4090
Uniprot:	Q99717
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	52kDa
Observed MW:	52KDa

Synonyms:	DWFC, JV5-1, MADH5, SMAD5
Background:	The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SMAD5: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor; DNA-binding Chromosomal Location of Human Ortholog: 5q31 Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; integral to membrane; intracellular; nucleus; cytosol Molecular Function:protein binding; ubiquitin protein ligase binding; metal ion binding; receptor signaling protein activity; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity Biological Process: Mullerian duct regression; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; embryonic pattern specification; signal transduction; protein amino acid phosphorylation; germ cell development; BMP signaling pathway; positive regulation of osteoblast differentiation; ureteric bud development; transforming growth factor beta receptor signaling pathway; cartilage development; erythrocyte differentiation; cardiac muscle contraction; osteoblast fate commitment
NCBI Summary:	The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Code:	Q99717
NCBI GenInfo Identifier:	13959566
NCBI Gene ID:	4090
NCBI Accession:	Q99717.1
UniProt Secondary Accession:	Q99717,O14688, Q15798, Q9UQA1,
UniProt Related Accession:	Q99717
Molecular Weight:	52,258 Da
NCBI Full Name:	Mothers against decapentaplegic homolog 5
NCBI Synonym Full Names:	SMAD family member 5
NCBI Official Symbol:	SMAD5
NCBI Official Synonym Symbols:	DWFC; JV5-1; MADH5
NCBI Protein Information:	mothers against decapentaplegic homolog 5; SMA- and MAD-related protein 5; SMAD, mothers against DPP homolog 5; MAD, mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5
UniProt Protein Name:	Mothers against decapentaplegic homolog 5
UniProt Synonym Protein Names:	JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5
Protein Family:	SMAD5 antisense gene protein
UniProt Gene Name:	SMAD5
UniProt Entry Name:	SMAD5_HUMAN

	Western blot analysis of extracts of Rat lung, using Smad5 antibody (CAB0635) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded mouse brain using SMAD5 antibody (CAB0635) (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using SMAD5 antibody (CAB0635) (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using SMAD5 antibody (CAB0635) (40x lens).

Antibodies	ELISA Kits
Anti-Smad5 Antibody (CAB14023)	Human Mothers against decapentaplegic homolog 5 (SMAD5) ELISA Kit
Anti-Smad5 Antibody (CAB1947)
Anti-Smad5 Antibody [KO Validated] (CAB19117)
Anti-Phospho-Smad5-S463/S465 Antibody (CABP1023)
Anti-Phospho-Smad5-S463/465 Antibody (CABP1130)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)