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Western blot analysis of extracts of various cell lines, using SMARCB1/SNF5 antibody (CAB21826) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

SMARCB1/SNF5 Polyclonal Antibody (CAB21826)

Datasheet MSDS
SKU CAB21826
Product Type Antibody
Antibody Type Polyclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms RDT, CSS3, INI1, SNF5, Snr1, BAF47, INI-1, MRD15, RTPS1, Sfh1p, hSNFS, SNF5L1, SWNTS1, PPP1R144, SMARCB1/SNF5
€96 - €370

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Description

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SMARCB1/SNF5 Polyclonal Antibody (CAB21826)

The SMARCB1/SNF5 Polyclonal Antibody (CAB21826) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to SMARCB1, this antibody enables the detection and analysis of the protein in various cell types, making it well-suited for studies in cancer biology and epigenetics.SMARCB1 plays a crucial role in the regulation of gene expression by modulating chromatin structure, thereby influencing various cellular processes such as proliferation, differentiation, and DNA repair.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SMARCB1/SNF5 Polyclonal Antibody
SKU:CAB21826
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:LWRR LATV EERK KIVA SSHG KKTK PNTK DHGY TTLA TSVT LLKA SEVE EILD GNDE KYKA VSIS TEPP TYLR EQKA KRNS QWVP TLPN SSHH LDAV PCST T
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RDT, CSS3, INI1, SNF5, Snr1, BAF47, INI-1, MRD15, RTPS1, Sfh1p, hSNFS, SNF5L1, SWNTS1, PPP1R144, SMARCB1/SNF5
Positive Sample:SH-SY5Y, THP-1, Hep G2, HeLa, Mouse testis, Rat testis
Cellular Localization:Nucleus.
Calculated MW:44kDa
Observed MW:Refertofigures

The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene.

Purification MethodAffinity purification
Gene ID6598
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using SMARCB1/SNF5 Rabbit pAb (CAB21826) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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