Anti-STC1 Antibody (CAB6755)

Antibody Name:	Anti-STC1 Antibody
Antibody SKU:	CAB6755
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 18-247 of human STC1 (NP_003146.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 18-247 of human STC1 (NP_003146.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	THEA EQND SVSP RKSR VAAQ NSAE VVRC LNSA LQVG CGAF ACLE NSTC DTDG MYDI CKSF LYSA AKFD TQGK AFVK ESLK CIAN GVTS KVFL AIRR CSTF QRMI AEVQ EECY SKLN VCSI AKRN PEAI TEVV QLPN HFSN RYYN RLVR SLLE CDED TVST IRDS LMEK IGPN MASL FHIL QTDH CAQT HPRA DFNR RRTN EPQK LKVL LRNL RGEE DSPS HIKR TSHE SA
Gene ID:	6781
Uniprot:	P52823
Cellular Location:	Secreted
Calculated MW:	20kDa/27kDa
Observed MW:	28kDa

Synonyms:

STC1, STC

Background:

This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The gene contains a 5' UTR rich in CAG trinucleotide repeats. The encoded protein contains 11 conserved cysteine residues and is phosphorylated by protein kinase C exclusively on its serine residues. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Overexpression of human stanniocalcin 1 in mice produces high serum phosphate levels, dwarfism, and increased metabolic rate. This gene has altered expression in hepatocellular, ovarian, and breast cancers.

UniProt Protein Function:	STC1: Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia. Belongs to the stanniocalcin family.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 8p21.2 Cellular Component: extracellular space; cytoplasm; apical plasma membrane; nucleus Molecular Function:hormone activity Biological Process: cellular calcium ion homeostasis; negative regulation of calcium ion transport; ossification; response to vitamin D; endothelial cell morphogenesis; decidualization; negative regulation of cell migration; embryo implantation
NCBI Summary:	This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The gene contains a 5' UTR rich in CAG trinucleotide repeats. The encoded protein contains 11 conserved cysteine residues and is phosphorylated by protein kinase C exclusively on its serine residues. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Overexpression of human stanniocalcin 1 in mice produces high serum phosphate levels, dwarfism, and increased metabolic rate. This gene has altered expression in hepatocellular, ovarian, and breast cancers. [provided by RefSeq, Jul 2008]
UniProt Code:	P52823
NCBI GenInfo Identifier:	1706175
NCBI Gene ID:	6781
NCBI Accession:	P52823.1
UniProt Secondary Accession:	P52823,Q71UE5,
UniProt Related Accession:	P52823
Molecular Weight:
NCBI Full Name:	Stanniocalcin-1
NCBI Synonym Full Names:	stanniocalcin 1
NCBI Official Symbol:	STC1
NCBI Official Synonym Symbols:	STC
NCBI Protein Information:	stanniocalcin-1
UniProt Protein Name:	Stanniocalcin-1
Protein Family:	Stanniocalcin
UniProt Gene Name:	STC1
UniProt Entry Name:	STC1_HUMAN

	Western blot analysis of extracts of mouse lung, using STC1 Antibody (CAB6755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using STC1 antibody (CAB6755) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver using STC1 antibody (CAB6755) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using STC1 antibody (CAB6755) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using STC1 antibody (CAB6755) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-STC1 Antibody (CAB16976)	Human STC1 (Stanniocalcin-1) ELISA Kit (HUFI06304)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)