Streptavidin-NC Recombinant Protein (RPPB4859)
- SKU:
- RPPB4859
- Product Type:
- Recombinant Protein
Description
| Product Name: | Streptavidin-NC Recombinant Protein |
| Product Code: | RPPB4859 |
| Size: | 100µg |
| Target: | Streptavidin-NC |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Liquid formulation. |
| Formulation: | The sterile solution contains 10mM K2HPO4-KH2PO4, pH 7.3. |
| Stability: | Streptavidin-NC although stable at 4°C for 3 weeks, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Purity: | Greater than 94.0% as determined by SDS-PAGE. |
Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.
Recombinant Streptavidin-NC produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 24kDa. Streptavidin-NC is engineered to bind to nitrocellulose.
