Streptavidin Recombinant Protein (RPPB4857)
- SKU:
- RPPB4857
- Product Type:
- Recombinant Protein
- Uniprot:
- P22629
Description
| Product Name: | Streptavidin Recombinant Protein |
| Product Code: | RPPB4857 |
| Size: | 20µg |
| Target: | Streptavidin |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The Streptavidin protein solution (1mg/ml) contains 20mM Tris-HCl pH7.5. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by analysis by SDS-PAGE. |
| Amino Acid Sequence: | MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ |
Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is widely used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.
Recombinant Streptomyces Avidinii Streptavidin produced in E.Coli is a single, non-glycosylated polypeptide chain (25-183) containing a total of 167 amino acids and having a molecular mass of 17kDa. The Streptavidin protein is fused to an 8 aa N-terminal His-Tag and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | streptavidin: The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). (organism: Streptomyces avidinii) |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P22629 |
| NCBI GenInfo Identifier: | 134951 |
| NCBI Gene ID: | |
| NCBI Accession: | P22629.1 |
| UniProt Secondary Accession: | P22629 |
| UniProt Related Accession: | P22629 |
| Molecular Weight: | |
| NCBI Full Name: | Streptavidin |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | |
| UniProt Protein Name: | Streptavidin |
| UniProt Synonym Protein Names: | |
| Protein Family: | Streptavidin |
| UniProt Gene Name: | |
| UniProt Entry Name: | SAV_STRAV |
