Anti-TCEB2 Antibody (CAB13363)

Antibody Name:	Anti-TCEB2 Antibody
Antibody SKU:	CAB13363
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human TCEB2 (NP_009039.1).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human
Positive Samples:	22Rv1, NCI-H460, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human TCEB2 (NP_009039.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDVF LMIR RHKT TIFT DAKE SSTV FELK RIVE GILK RPPD EQRL YKDD QLLD DGKT LGEC GFTS QTAR PQAP ATVG LAFR ADDT FEAL CIEP FSSP PELP DVMK PQDS GSSA NEQA VQ
Gene ID:	6923
Uniprot:	Q15370
Cellular Location:	Nucleus
Calculated MW:	13kDa/17kDa
Observed MW:	16kDa

Synonyms:

ELOB, SIII, TCEB2, elongin-B

Background:

This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.

UniProt Protein Function:	SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (PubMed:7638163). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells.
NCBI Summary:	This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13. [provided by RefSeq, Aug 2008]
UniProt Code:	Q15370
NCBI GenInfo Identifier:	32699512
NCBI Gene ID:	6923
NCBI Accession:	Q15370.1
UniProt Related Accession:	Q15370
Molecular Weight:
NCBI Full Name:	Elongin-B
NCBI Synonym Full Names:	elongin B
NCBI Official Symbol:	ELOB
NCBI Official Synonym Symbols:	SIII; TCEB2
NCBI Protein Information:	elongin-B
UniProt Protein Name:	Elongin-B
UniProt Synonym Protein Names:	Elongin 18 kDa subunit; RNA polymerase II transcription factor SIII subunit B; SIII p18; Transcription elongation factor B polypeptide 2
UniProt Gene Name:	ELOB

	Western blot analysis of extracts of various cell lines, using TCEB2 antibody (CAB13363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon using TCEB2 antibody (CAB13363) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using TCEB2 antibody (CAB13363) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using TCEB2 antibody (CAB13363) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug TCEB2 antibody (CAB13363). Western blot was performed from the immunoprecipitate using TCEB2 antibody (CAB13363) at a dilition of 1:500.

Antibodies

Anti-TCEB2 Antibody (CAB5362)

TCEB2 Antibody (PACO12669)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)