Western blot analysis of extracts of various cell lines, using TCF7L1 antibody (CAB9006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The TCF7L1 Polyclonal Antibody (CAB9006) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity for human samples, making it suitable for use in Western blotting and immunofluorescence applications.TCF7L1, also known as T-cell factor 7 like 1 or TCF3, is a crucial regulator of gene expression during early embryogenesis and plays a role in maintaining stem cell populations. Dysregulation of TCF7L1 has been implicated in various diseases, including cancer and developmental disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TCF7L1 Polyclonal Antibody
SKU:
CAB9006
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TCF3, TCF-3, TCF7L1
Positive Sample:
HeLa, HCT116, A-549, A-431, Mouse lung, Mouse brain, Mouse skin, Rat lung, Rat skin
Cellular Localization:
Nucleus.
Calculated MW:
63kDa
Observed MW:
75kDa
This gene encodes a member of the T cell factor/lymphoid enhancer factor family of transcription factors. These transcription factors are activated by beta catenin, mediate the Wnt signaling pathway and are antagonized by the transforming growth factor beta signaling pathway. The encoded protein contains a high mobility group-box DNA binding domain and participates in the regulation of cell cycle genes and cellular senescence.
Purification Method
Affinity purification
Gene ID
83439
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TCF7L1 Rabbit pAb (CAB9006) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
