Anti-TRAF4 Antibody (CAB7047)

Antibody Name:	Anti-TRAF4 Antibody
Antibody SKU:	CAB7047
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 251-470 of human TRAF4 (NP_004286.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 251-470 of human TRAF4 (NP_004286.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PFKD SGCK HRCP KLAM ARHV EESV KPHL AMMC ALVS RQRQ ELQE LRRE LEEL SVGS DGVL IWKI GSYG RRLQ EAKA KPNL ECFS PAFY THKY GYKL QVSA FLNG NGSG EGTH LSLY IRVL PGAF DNLL EWPF ARRV TFSL LDQS DPGL AKPQ HVTE TFHP DPNW KNFQ KPGT WRGS LDES SLGF GYPK FISH QDIR KRNY VRDD AVFI RAAV ELPR KILS
Gene ID:	9618
Uniprot:	Q9BUZ4
Cellular Location:	Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus, Peripheral membrane protein, cytoskeleton, perinuclear region, tight junction
Calculated MW:	22kDa/53kDa
Observed MW:	54kDa

Synonyms:

TRAF4, CART1, MLN62, RNF83

Background:

This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined.

UniProt Protein Function:	TRAF4: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract. Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. Belongs to the TNF receptor-associated factor family. B subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; Adaptor/scaffold Chromosomal Location of Human Ortholog: 17q11-q12 Cellular Component: cytoplasm; nucleus Molecular Function:DNA binding; protein binding; thioesterase binding; tumor necrosis factor receptor binding; ubiquitin protein ligase binding; WW domain binding Biological Process: positive regulation of JNK cascade; positive regulation of protein kinase activity; signal transduction
NCBI Summary:	This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9BUZ4
NCBI GenInfo Identifier:	30580636
NCBI Gene ID:	9618
NCBI Accession:	Q9BUZ4.1
UniProt Secondary Accession:	Q9BUZ4,O75615, Q14848, Q2KJU4, Q2PJN8,
UniProt Related Accession:	Q9BUZ4
Molecular Weight:	22,841 Da
NCBI Full Name:	TNF receptor-associated factor 4
NCBI Synonym Full Names:	TNF receptor associated factor 4
NCBI Official Symbol:	TRAF4
NCBI Official Synonym Symbols:	CART1; MLN62; RNF83
NCBI Protein Information:	TNF receptor-associated factor 4
UniProt Protein Name:	TNF receptor-associated factor 4
UniProt Synonym Protein Names:	Cysteine-rich domain associated with RING and Traf domains protein 1; Metastatic lymph node gene 62 protein; MLN 62; RING finger protein 83
Protein Family:	TNF receptor-associated factor
UniProt Gene Name:	TRAF4
UniProt Entry Name:	TRAF4_HUMAN

	Western blot analysis of extracts of various cell lines, using TRAF4 antibody (CAB7047) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using TRAF4 antibody (CAB7047).

Antibodies

TRAF4 Antibody (PACO05793)

TRAF4 Antibody (PACO12884)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)