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Anti-TRIB3 Antibody (CAB5424)

SKU:
CAB5424
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-TRIB3 Antibody
Antibody SKU:CAB5424
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human TRIB3 (NP_066981.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:293T, SK-HEP1, K-562, SKOV3, BT-474, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human TRIB3 (NP_066981.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRAT PLAA PAGS LSRK KRLE LDDN LDTE RPVQ KRAR SGPQ PRLP PCLL PLSP PTAP DRAT AVAT ASRL GPYV LLEP EEGG RAYQ ALHC PTGT EYTC KVYP VQEA LAVL EPYA RLPP HKHV ARPT EVLA GT
Gene ID:57761
Uniprot:Q96RU7
Cellular Location:Nucleus
Calculated MW:39kDa
Observed MW:35kDa
Synonyms:TRIB3, C20orf97, NIPK, SINK, SKIP3, TRB3
Background:The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants.
UniProt Protein Function:TRB3: a Ca(2)/calmodulin-dependent protein kinase of the Trbl family. Induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. May negatively regulate the cell survival kinase AKT1.
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; Trbl family

Chromosomal Location of Human Ortholog: 20p13-p12.2

Cellular Component: nucleoplasm; plasma membrane; cytosol; nucleus

Molecular Function:protein binding; protein kinase inhibitor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity; kinase activity; transcription corepressor activity; protein kinase binding; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; transcription, DNA-dependent; nerve growth factor receptor signaling pathway; positive regulation of protein binding; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; protein amino acid phosphorylation; cellular response to insulin stimulus; regulation of MAP kinase activity; positive regulation of ubiquitin-protein ligase activity; insulin receptor signaling pathway; negative regulation of protein kinase activity; innate immune response; negative regulation of transcription, DNA-dependent; negative regulation of fatty acid biosynthetic process

NCBI Summary:The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Code:Q96RU7
NCBI GenInfo Identifier:28201830
NCBI Gene ID:57761
NCBI Accession:Q96RU7.2
UniProt Related Accession:Q96RU7
Molecular Weight:35kDa
NCBI Full Name:Tribbles homolog 3
NCBI Synonym Full Names:tribbles pseudokinase 3
NCBI Official Symbol:TRIB3  
NCBI Official Synonym Symbols:NIPK; SINK; TRB3; SKIP3; C20orf97  
NCBI Protein Information:tribbles homolog 3
UniProt Protein Name:Tribbles homolog 3
UniProt Synonym Protein Names:Neuronal cell death-inducible putative kinase; SINK; p65-interacting inhibitor of NF-kappa-B
Protein Family:Tribbles
UniProt Gene Name:TRIB3  
UniProt Entry Name:TRIB3_HUMAN
Anti-TRIB3 Antibody (CAB5424)Western blot analysis of extracts of various cell lines, using TRIB3 antibody (CAB5424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.

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