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Anti-Alpha-Tubulin Antibody (CABC007)

SKU:
CABC007
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
  • Loading Controls Anti-Alpha-Tubulin Antibody CABC007
  • Loading Controls Anti-Alpha-Tubulin Antibody CABC007
  • Loading Controls Anti-Alpha-Tubulin Antibody CABC007
  • Loading Controls Anti-Alpha-Tubulin Antibody CABC007
  • Loading Controls Anti-Alpha-Tubulin Antibody CABC007
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Description

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Antibody Name:Anti-Alpha-Tubulin Antibody
Antibody SKU:CABC007
Antibody Size:100 uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human Alpha-Tubulin (NP_005991.1).
Application:WB IF
Recommended Dilution:WB 1:2000 - 1:10000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-431, HeLa, K-562, Mouse kidney, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human Alpha-Tubulin (NP_005991.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MREC ISVH VGQA GVQM GNAC WELY CLEH GIQP DGQM PSDK TIGG GDDS FTTF FCET GAGK HVPR AVFV DLEP TVID EIRN GPYR QLFH PEQL ITGK EDAA NNYA RGHY TIGK EIID PVLD RIRK LSDQ CTGL QGFL VFHS FGGG TGSG FTSL LMER LSVD YGKK SKLE FSIY PAPQ VSTA
Gene ID:7277
Uniprot:P68366
Cellular Location:Cytoplasm, cytoskeleton
Calculated MW:48kDa/49kDa
Observed MW:50kDa
Anti-Alpha-Tubulin Antibody (CABC007)Western blot analysis of extracts of various cell lines, using alpha-Tubulin antibody (CABC007) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-Alpha-Tubulin Antibody (CABC007)Immunofluorescence analysis of C6 cells using alpha-Tubulin Rabbit pAb (CABC007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-Alpha-Tubulin Antibody (CABC007)Immunofluorescence analysis of NIH-3T3 cells using alpha-Tubulin Rabbit pAb (CABC007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-Alpha-Tubulin Antibody (CABC007)Immunofluorescence analysis of U-2 OS cells using Alpha-Tubulin Rabbit pAb (CABC007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-Alpha-Tubulin Antibody (CABC007)Confocal immunofluorescence analysis of U2OS cells using Alpha-Tubulin antibody (CABC007) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.
Synonyms:TUBA4A, ALS22, H2-ALPHA, TUBA1
Background:Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:TUBA4A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: microtubule; extracellular region; cytosol

Molecular Function:GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; platelet activation; cellular protein metabolic process; platelet degranulation; protein folding; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; microtubule-based process

Disease: Amyotrophic Lateral Sclerosis 22 With Or Without Frontotemporal Dementia

NCBI Summary:Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]
UniProt Code:P68366
NCBI GenInfo Identifier:55977476
NCBI Gene ID:7277
NCBI Accession:P68366.1
UniProt Related Accession:P68366
Molecular Weight:50kDa
NCBI Full Name:Tubulin alpha-4A chain
NCBI Synonym Full Names:tubulin alpha 4a
NCBI Official Symbol:TUBA4A  
NCBI Official Synonym Symbols:ALS22; TUBA1; H2-ALPHA  
NCBI Protein Information:tubulin alpha-4A chain
UniProt Protein Name:Tubulin alpha-4A chain
UniProt Synonym Protein Names:Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain
Protein Family:Tubulin
UniProt Gene Name:TUBA4A  
UniProt Entry Name:TBA4A_HUMAN

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