Anti-Alpha-Tubulin Antibody (CABC014)
- SKU:
- CABC014
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Frequently bought together:
Description
| Antibody Name: | Anti-Alpha-Tubulin Antibody |
| Antibody SKU: | CABC014 |
| Antibody Size: | 100 uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human Alpha-Tubulin |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:25 - 1:50 IF 1:25 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, MCF7, PC-12, Mouse heart, Mouse brain |
| Immunogen: | Recombinant protein of human Alpha-Tubulin |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 7277 |
| Uniprot: | P68366 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 48kDa/49kDa |
| Observed MW: | 55kDa |
 | Western blot analysis of extracts of various cell lines, using a-Tubulin Rabbit pAb (CABC014) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
 | Immunohistochemistry of paraffin-embedded rat brain using alpha-Tubulin Antibody (CABC014) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using Alpha-Tubulin Antibody (CABC014) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using Alpha-Tubulin Antibody (CABC014) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using Alpha-Tubulin antibody (CABC014). Blue: DAPI for nuclear staining. |
| Synonyms: | TUBA4A, ALS22, H2-ALPHA, TUBA1 |
| Background: | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | TUBA4A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 2q35 Cellular Component: microtubule; extracellular region; cytosol Molecular Function:GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton Biological Process: protein polymerization; 'de novo' posttranslational protein folding; platelet activation; cellular protein metabolic process; platelet degranulation; protein folding; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; microtubule-based process Disease: Amyotrophic Lateral Sclerosis 22 With Or Without Frontotemporal Dementia |
| NCBI Summary: | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] |
| UniProt Code: | P68366 |
| NCBI GenInfo Identifier: | 55977476 |
| NCBI Gene ID: | 7277 |
| NCBI Accession: | P68366.1 |
| UniProt Related Accession: | P68366 |
| Molecular Weight: | 50kDa |
| NCBI Full Name: | Tubulin alpha-4A chain |
| NCBI Synonym Full Names: | tubulin alpha 4a |
| NCBI Official Symbol: | TUBA4A |
| NCBI Official Synonym Symbols: | ALS22; TUBA1; H2-ALPHA |
| NCBI Protein Information: | tubulin alpha-4A chain |
| UniProt Protein Name: | Tubulin alpha-4A chain |
| UniProt Synonym Protein Names: | Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain |
| Protein Family: | Tubulin |
| UniProt Gene Name: | TUBA4A |
| UniProt Entry Name: | TBA4A_HUMAN |
Related Products
Anti-Alpha-Tubulin Antibody (CABC007)
€279
Anti-alpha-Tubulin Antibody (CAB21092)
€139 - €469
Anti-alpha-Tubulin Antibody (CAB6830)
€159 - €419
Anti-Alpha-Tubulin Antibody (CABC031)
€279
Anti-TUBB3 Antibody (MACO0178)
€439
