Anti-Alpha-Tubulin Antibody (CABC031)

SKU:
CABC031
€229

Description

Technical Manual

Anti-Alpha-Tubulin Antibody

Product Name:Anti-Alpha-Tubulin Antibody
Product SKU:CABC031
Size:100 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen:Recombinant protein of human Alpha-Tubulin
Sequence:

Email for sequence

Gene ID:7277
Swiss Prot:P68366
Synonyms:TUBA4A; ALS22; H2-ALPHA; TUBA1
Calculated MW:48kDa/49kDa
Observed MW:55kDa
Reactivity:Human, Mouse, Rat
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry 
Positive Samples:HeLa, Jurkat, A-431, Mouse brain, Mouse lung, Rat brain
Cellular Location:Cytoplasm, cytoskeleton

Product Images

Anti-Alpha-Tubulin Antibody (CABC031)

Western blot analysis of extracts of various cell lines, using alpha-Tubulin antibody (CABC031) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Uniprot Information

UniProt Protein Function:TUBA4A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: microtubule; extracellular region; cytosol

Molecular Function:GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; platelet activation; cellular protein metabolic process; platelet degranulation; protein folding; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; microtubule-based process

Disease: Amyotrophic Lateral Sclerosis 22 With Or Without Frontotemporal Dementia

NCBI Summary:Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]
UniProt Code:P68366
NCBI GenInfo Identifier:55977476
NCBI Gene ID:7277
NCBI Accession:P68366.1
UniProt Related Accession:P68366
Molecular Weight:50kDa
NCBI Full Name:Tubulin alpha-4A chain
NCBI Synonym Full Names:tubulin alpha 4a
NCBI Official Symbol:TUBA4A 
NCBI Official Synonym Symbols:ALS22; TUBA1; H2-ALPHA 
NCBI Protein Information:tubulin alpha-4A chain
UniProt Protein Name:Tubulin alpha-4A chain
UniProt Synonym Protein Names:Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain
Protein Family:Tubulin
UniProt Gene Name:TUBA4A 
UniProt Entry Name:TBA4A_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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