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Anti-UBE2I Antibody (CAB13558)

SKU:
CAB13558
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 1 Anti-UBE2I Antibody CAB13558
  • Cell Cycle Antibodies 1 Anti-UBE2I Antibody CAB13558
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-UBE2I Antibody
Antibody SKU:CAB13558
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human UBE2I (NP_003336.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:SW620
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human UBE2I (NP_003336.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSGI ALSR LAQE RKAW RKDH PFGF VAVP TKNP DGTM NLMN WECA IPGK KGTP WEGG LFKL RMLF KDDY PSSP PKCK FEPP LFHP NVYP SGTV CLSI LEED KDWR PAIT IKQI LLGI QELL NEPN IQDP AQAE AYTI YCQN RVEY EKRV RAQA KKFA PS
Gene ID:7329
Uniprot:P63279
Cellular Location:Cytoplasm, Nucleus
Calculated MW:18kDa
Observed MW:18kDa
Synonyms:UBE2I, C358B7.1, P18, UBC9
Background:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:Function: E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2). Ref.4 Ref.5
UniProt Protein Details:

Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.

Pathway: Protein modification; protein sumoylation.

Subunit structure: Interacts with SIZ1. Ref.5 Ref.6

Subcellular location: Nucleus Ref.7.

Miscellaneous: Present with 2600 molecules/cell in log phase SD medium. Ref.8

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.

UniProt Code:P63279
NCBI GenInfo Identifier:
NCBI Gene ID:
NCBI Accession:
Molecular Weight:17,911 Da
NCBI Full Name:UBC9
UniProt Protein Name:SUMO-conjugating enzyme UBC9
UniProt Synonym Protein Names:Ubiquitin carrier protein 9; Ubiquitin-conjugating enzyme E2-18 kDa; Ubiquitin-protein ligase
UniProt Gene Name:UBC9  
UniProt Entry Name:UBC9_YEAST
Anti-UBE2I Antibody (CAB13558)Western blot analysis of extracts of SW620 cells, using UBE2I Rabbit pAb (CAB13558) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
Anti-UBE2I Antibody (CAB13558)Immunofluorescence analysis of U2OS cells using UBE2I antibody (CAB13558). Blue: DAPI for nuclear staining.

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