The Ubiquitin Rabbit Monoclonal Antibody (CAB19686) is a valuable tool for researchers studying ubiquitin, a small regulatory protein that plays a critical role in protein degradation, DNA repair, and cell signaling pathways. This antibody, produced in rabbits, is highly specific for ubiquitin and has been validated for use in Western blot and immunohistochemistry applications.Ubiquitin is involved in various cellular processes, including protein turnover and quality control, making it a key target for investigation in cancer research, neurodegenerative diseases, and the immune response. The Ubiquitin Rabbit Monoclonal Antibody allows for the detection and analysis of ubiquitin in different cell types, providing researchers with a powerful tool for studying its functions and interactions in various biological contexts.
By understanding the role of ubiquitin in cellular processes, researchers can gain insights into disease mechanisms and potentially identify new therapeutic targets for the treatment of various disorders. The Ubiquitin Rabbit Monoclonal Antibody is a reliable reagent that can contribute to advancing our knowledge of ubiquitin biology and its implications in health and disease.
Product Name:
Ubiquitin Rabbit Monoclonal Antibody
SKU:
CAB19686
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human Ubiquitin (AAH46123.1).
HeLa treated by MG132,NIH/3T3 treated by MG132,C6 treated by MG132
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
26kDa
Observed MW:
15-300kDa
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
7314
Clone Number:
ARC50024
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Ubiquitin Rabbit mAb (CAB19686) at1:1000 dilution.HeLa,NIH/3T3 and C6 cell were treated by MG132(50 μM) at 37℃ for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
