The Ubiquitin Polyclonal Antibody (CAB18185) is a valuable tool for research involving ubiquitin, a small regulatory protein critical for various cellular processes including protein degradation, DNA repair, and signal transduction. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Ubiquitin plays a crucial role in targeting proteins for degradation by the proteasome, as well as in regulating protein stability, localization, and function. Dysregulation of the ubiquitin system has been implicated in various diseases, including cancer, neurodegenerative disorders, and infectious diseases.
By targeting ubiquitin in research, scientists can gain insights into the mechanisms underlying these diseases and potentially identify therapeutic targets.The Ubiquitin Polyclonal Antibody is a reliable tool for studying the ubiquitin system in various cell types and biological contexts. Its high specificity and sensitivity make it suitable for a wide range of research applications, including immunofluorescence, immunohistochemistry, and flow cytometry. Researchers interested in understanding the role of ubiquitin in health and disease will find this antibody essential for their studies.
Product Name:
Ubiquitin Rabbit Polyclonal Antibody
SKU:
CAB18185
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human Ubiquitin (NP_061828.1).
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
7314
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells using Ubiquitin Rabbit pAb(CAB18185) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s.
