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Anti-USP33 Antibody (CAB16187)

SKU:
CAB16187
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 6 Anti-USP33 Antibody CAB16187
  • Cell Biology Antibodies 6 Anti-USP33 Antibody CAB16187
  • Cell Biology Antibodies 6 Anti-USP33 Antibody CAB16187
  • Cell Biology Antibodies 6 Anti-USP33 Antibody CAB16187
  • Cell Biology Antibodies 6 Anti-USP33 Antibody CAB16187
€139 - €419
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Description

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Antibody Name:Anti-USP33 Antibody
Antibody SKU:CAB16187
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 290-400 of human USP33 (NP_963920.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 290-400 of human USP33 (NP_963920.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QVME VEED PQTI TTEE TMEE DKSQ SDVD FQSC ESCS NSDR AENE NGSR CFSE DNNE TTML IQDD ENNS EMSK DWQK EKMC NKIN KVNS EGEF DKDR DSIS ETVD LNNQ ETV
Gene ID:23032
Uniprot:Q8TEY7
Cellular Location:Cytoplasm, Golgi apparatus, centrosome, cytoskeleton, microtubule organizing center, perinuclear region
Calculated MW:93kDa/103kDa/106kDa
Observed MW:107KDa
Synonyms:USP33, VDU1
Background:This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms.
NCBI Summary:This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]
UniProt Code:Q8TEY7
NCBI GenInfo Identifier:42516567
NCBI Gene ID:23032
NCBI Accession:NP_055832.3
UniProt Secondary Accession:Q8TEY7,A6QNM7, Q8TEY6,
UniProt Related Accession:Q8TEY7
Molecular Weight:
NCBI Full Name:ubiquitin carboxyl-terminal hydrolase 33 isoform 1
NCBI Synonym Full Names:ubiquitin specific peptidase 33
NCBI Official Symbol:USP33  
NCBI Official Synonym Symbols:VDU1  
NCBI Protein Information:ubiquitin carboxyl-terminal hydrolase 33
UniProt Protein Name:Ubiquitin carboxyl-terminal hydrolase 33
UniProt Synonym Protein Names:Ubiquitin thiolesterase 33; Ubiquitin-specific-processing protease 33; Deubiquitinating enzyme 33; VHL-interacting deubiquitinating enzyme 1
UniProt Gene Name:USP33  
UniProt Entry Name:UBP33_HUMAN
Anti-USP33 Antibody (CAB16187)Western blot analysis of extracts of HeLa cells, using USP33 Polyclonal Antibody (CAB16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min.
Anti-USP33 Antibody (CAB16187)Western blot analysis of extracts of HepG2 cells, using USP33 Polyclonal Antibody (CAB16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Anti-USP33 Antibody (CAB16187)Immunohistochemistry of paraffin-embedded human breast cancer using USP33 antibody (CAB16187) at dilution of 1:100 (40x lens).
Anti-USP33 Antibody (CAB16187)Immunohistochemistry of paraffin-embedded rat ovary using USP33 antibody (CAB16187) at dilution of 1:100 (40x lens).
Anti-USP33 Antibody (CAB16187)Immunofluorescence analysis of NIH-3T3 cells using USP33 Polyclonal Antibody (CAB16187) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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