Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-VMP1 Polyclonal Antibody (STPA00068). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody (STPA00068) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT.
VMP1 Antibody is a rabbit polyclonal antibody directed against VMP1, offered by Assay Genie for research applications. Reported applications include WB and ICC/IF. Reported reactivity: Human. Purified by Peptide Affinity.
Vacuole Membrane Protein 1 (VMP1) is an endoplasmic reticulum (ER)-localized, multi-spanning membrane protein essential for the initiation of autophagy and the maintenance of ER-organelle communication. VMP1 plays a pivotal role in forming autophagosomes and regulating membrane contact sites between the ER and other organelles, including mitochondria, endosomes, and lipid droplets. In the nervous system, VMP1 is crucial for neuronal homeostasis. It facilitates the clearance of damaged proteins and organelles through the autophagy-lysosome pathway—a process vital for long-lived neurons. Loss of VMP1 function disrupts autophagic flux, leading to the accumulation of protein aggregates, damaged mitochondria, and swollen ER structures. These cellular stressors are strongly associated with the pathogenesis of neurodegenerative diseases such as Parkinson’s disease, Alzheimer’s disease, and amyotrophic lateral sclerosis. Recent studies have shown that VMP1 deficiency in dopaminergic neurons results in progressive motor deficits, axonal degeneration, and α-synuclein accumulation—hallmarks of Parkinsonian neurodegeneration. Mechanistically, VMP1 is required for LC3 recruitment and Beclin-1 interaction, both essential steps in autophagosome formation. As a master regulator of autophagy and ER-organelle dynamics, VMP1 represents a promising therapeutic target for neurodegenerative disease intervention. Its role in maintaining neuronal integrity positions it at the forefront of research into brain aging and neuroprotection. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, ICC/IF
Purification:
Peptide Affinity Purified
Concentration:
1 mg/ml
Reactivity:
Human
Recommended Dilution:
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Specificity:
Detects ~46 kDa.
Guarantee:
12 months from date of dispatch
Immunogen:
Synthetic peptide from the C-terminal of Human VMP1 (aa. 391-402)
Immunogen Species:
Human
Accession Number:
NP_112200.2
Gene ID:
81671
Swiss-Prot:
Q96GC9
Cellular Localization:
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane | Multi-Pass Membrane Protein | Cell Membrane | Multi-Pass Membrane Protein | Vacuole Membrane | Multi-Pass Membrane Protein | Endoplasmic Reticulum
Alternative Names:
VMP1, Vacuole Membrane Protein 1, TMEM49, Transmembrane Protein 49, TANGO5, Transport And Golgi Organization 5 Homolog, EPG3, Ectopic P-Granules Autophagy Protein 3 Homolog, TDC1, HSPC292
