Anti-VSNL1 Antibody (CAB6999)

Antibody Name:	Anti-VSNL1 Antibody
Antibody SKU:	CAB6999
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human VSNL1 (NP_003376.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, 5637, Mouse brain, Mouse lung, Rat lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human VSNL1 (NP_003376.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGKQ NSKL APEV MEDL VKST EFNE HELK QWYK GFLK DCPS GRLN LEEF QQLY VKFF PYGD ASKF AQHA FRTF DKNG DGTI DFRE FICA LSIT SRGS FEQK LNWA FNMY DLDG DGKI TRVE MLEI IEAI YKMV GTVI MMKM NEDG LTPE QRVD KIFS KMDK NKDD QITL DEFK EAAK SDPS IVLL LQCD IQK
Gene ID:	7447
Uniprot:	P62760
Cellular Location:
Calculated MW:	22kDa
Observed MW:	23kDa

Synonyms:

VSNL1, HLP3, HPCAL3, HUVISL1, VILIP, VILIP-1

Background:

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

UniProt Protein Function:	VSNL1: Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner. Belongs to the recoverin family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 2p24.3 Molecular Function:calcium ion binding Biological Process: calcium-mediated signaling
NCBI Summary:	This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	P62760
NCBI GenInfo Identifier:	51338696
NCBI Gene ID:	7447
NCBI Accession:	P62760.2
UniProt Secondary Accession:	P62760,P28677, P29103, P42323, Q9UM20, D6W515,
UniProt Related Accession:	P62760
Molecular Weight:	191
NCBI Full Name:	Visinin-like protein 1
NCBI Synonym Full Names:	visinin-like 1
NCBI Official Symbol:	VSNL1
NCBI Official Synonym Symbols:	HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1
NCBI Protein Information:	visinin-like protein 1; VLP-1; hippocalcin-like protein 3
UniProt Protein Name:	Visinin-like protein 1
UniProt Synonym Protein Names:	Hippocalcin-like protein 3; HLP3
Protein Family:	Visinin-like protein
UniProt Gene Name:	VSNL1
UniProt Entry Name:	VISL1_HUMAN

	Western blot analysis of extracts of various cell lines, using VSNL1 Antibody (CAB6999) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded mouse brain using VSNL1 antibody (CAB6999) at dilution of 1:200 (40x lens).

Antibodies	ELISA Kits
Anti-VSNL1 Antibody (CAB2797)	Human VILIP1 / VSNL1 ELISA Kit
	Human Visinin-like protein 1 (VSNL1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)