The XBP1s Polyclonal Antibody (AG10132) is a powerful tool for researchers studying the XBP1s protein, a key player in the unfolded protein response (UPR) pathway. Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the XBP1s protein, this antibody enables accurate detection and analysis in a variety of cell types, making it essential for studies in the fields of cellular stress, cancer biology, and neurodegenerative diseases.
XBP1s, a spliced form of X-box binding protein 1, is a crucial transcription factor that activates genes involved in ER stress response and protein folding. Dysregulation of the UPR pathway, in which XBP1s plays a central role, has been implicated in various diseases including cancer, neurodegenerative disorders, and metabolic diseases. By elucidating the function and regulation of XBP1s, researchers can gain valuable insights into disease mechanisms and potentially identify novel therapeutic targets for intervention.
Product Name:
XBP1s Rabbit Polyclonal Antibody
SKU:
CAB17007
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 277-376 of human XBP1s (NP_001073007.1).
Cytoplasm, Endoplasmic reticulum membrane, Endoplasmic reticulum, Membrane, Nucleus, Peripheral membrane protein, Single-pass type II membrane protein.
Calculated MW:
29kDa
Observed MW:
28kDa/50kDa
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
Purification Method:
Affinity purification
Gene ID:
7494
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using XBP1s Rabbit pAb (CAB17007) at 1:1000 dilution.HeLa cells were treated by Tunicamycin (2 μg/mL) for 8 hours.C6 cells were treated by Tunicamycin (2 μg/mL) for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
