The YWHAH Antibody (CAB9079) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific to human YWHAH samples, making it an ideal choice for Western blot applications. The YWHAH protein, also known as 14-3-3 eta, is involved in the regulation of diverse cellular functions by binding to phosphorylated serine/threonine residues on target proteins. Its role in cell signaling pathways makes it an important target for research in the fields of cancer biology, neurobiology, and cell biology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
YWHAH Antibody
SKU:
CAB9079
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder.
Purification Method
Affinity purification
Gene ID
7533
RRID
AB_2772935
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using YWHAH Rabbit pAb (CAB9079) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of NIH-3T3 cells using YWHAH Rabbit pAb (CAB9079) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
