Anti-A1CF Antibody (CAB13087)

Antibody Name:	Anti-A1CF Antibody
Antibody SKU:	CAB13087
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-120 of human A1CF (NP_055391.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, BxPC-3, HT-29, HeLa, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-120 of human A1CF (NP_055391.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	APPE RGCE IFIG KLPR DLFE DELI PLCE KIGK IYEM RMMM DFNG NNRG YAFV TFSN KVEA KNAI KQLN NYE
Gene ID:	29974
Uniprot:	Q9NQ94
Cellular Location:	Cytoplasm, Endoplasmic reticulum, Nucleus
Calculated MW:	55kDa/58kDa/62kDa/64kDa/65kDa
Observed MW:	65kDa

Synonyms:

A1CF, ACF, ACF64, ACF65, APOBEC1CF, ASP

Background:

Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene.

UniProt Protein Function:	ACF: Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; Hydrolase; RNA processing Chromosomal Location of Human Ortholog: 10q11.23 Cellular Component: apolipoprotein B mRNA editing enzyme complex; cytoplasm; nucleoplasm Molecular Function:double-stranded RNA binding; protein binding; RNA binding; single-stranded RNA binding Biological Process: cytidine to uridine editing; protein stabilization
NCBI Summary:	Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
UniProt Code:	Q9NQ94
NCBI GenInfo Identifier:	74761651
NCBI Gene ID:	29974
NCBI Accession:	Q9NQ94.1
UniProt Secondary Accession:	Q9NQ94,Q5SZQ0, Q9NQ93, Q9NQX8, Q9NQX9, Q9NXC9, Q9NZD3 A1L4F2, A8K7G7, B7ZM14,
UniProt Related Accession:	Q9NQ94
Molecular Weight:	58,871 Da
NCBI Full Name:	APOBEC1 complementation factor
NCBI Synonym Full Names:	APOBEC1 complementation factor
NCBI Official Symbol:	A1CF
NCBI Official Synonym Symbols:	ACF; ASP; ACF64; ACF65; APOBEC1CF
NCBI Protein Information:	APOBEC1 complementation factor
UniProt Protein Name:	APOBEC1 complementation factor
UniProt Synonym Protein Names:	APOBEC1-stimulating protein
Protein Family:	APOBEC1 complementation factor
UniProt Gene Name:	A1CF
UniProt Entry Name:	A1CF_HUMAN

	Western blot analysis of extracts of various cell lines, using A1CF antibody (CAB13087) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat ovary using A1CF antibody (CAB13087) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human tonsil using A1CF antibody (CAB13087) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using A1CF antibody (CAB13087) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)