Western blot analysis of various lysates, using ACAD8 Rabbit pAb (CAB23663) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The ACAD8 Polyclonal Antibody (CAB23663) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By binding to the ACAD8 protein, this antibody enables precise detection and analysis in various cell types, making it an essential tool for studies in metabolism and metabolic disorders.ACAD8 is a crucial enzyme that plays a key role in breaking down fatty acids for energy production. Dysregulation of ACAD8 activity has been implicated in various metabolic disorders, making it a promising target for research into obesity, diabetes, and other metabolic diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
ACAD8 Polyclonal Antibody
SKU:
CAB23663
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:7000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IBDH, ARC42, ACAD-8, ACAD8
Positive Sample:
Mouse liver, Rat kidney
Calculated MW:
45kDa
Observed MW:
43kDa
This gene encodes a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. The encoded protein is a mitochondrial enzyme that functions in catabolism of the branched-chain amino acid valine. Defects in this gene are the cause of isobutyryl-CoA dehydrogenase deficiency.
Purification Method
Affinity purification
Gene ID
27034
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ACAD8 Rabbit pAb (CAB23663) at 1:6000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
