Anti-EEF1A1 Antibody (CAB0974)

Antibody Name:	Anti-EEF1A1 Antibody
Antibody SKU:	CAB0974
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 123-462 of human EEF1A1 (NP_001393.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, MCF7, HeLa, Jurkat, HepG2, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 123-462 of human EEF1A1 (NP_001393.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FEAG ISKN GQTR EHAL LAYT LGVK QLIV GVNK MDST EPPY SQKR YEEI VKEV STYI KKIG YNPD TVAF VPIS GWNG DNML EPSA NMPW FKGW KVTR KDGN ASGT TLLE ALDC ILPP TRPT DKPL RLPL QDVY KIGG IGTV PVGR VETG VLKP GMVV TFAP VNVT TEVK SVEM HHEA LSEA LPGD NVGF NVKN VSVK DVRR GNVA GDSK NDPP MEAA GFTA QVII LNHP GQIS AGYA PVLD CHTA HIAC KFAE LKEK IDRR SGKK LEDG PKFL KSGD AAIV DMVP GKPM CVES FSDY PPLG RFAV RDMR QTVA VGVI KAVD KKAA GAGK VTKS AQKA QKAK
Gene ID:	1915
Uniprot:	P68104
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	47kDa/50kDa
Observed MW:	50kDa

Synonyms:

EEF1A1, CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, GRAF-1EF, HNGC:16303, LENG7, PTI1, eEF1A-1

Background:

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.

UniProt Protein Function:	EEF1A1: an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
UniProt Protein Details:	Protein type:Nucleolus; Translation; Translation elongation Chromosomal Location of Human Ortholog: 6q14.1 Cellular Component: extracellular space; cortical actin cytoskeleton; eukaryotic translation elongation factor 1 complex; membrane; cytoplasm; nucleolus; nucleus; cytosol Molecular Function:GTPase activity; protein binding; GTP binding; protein kinase binding; translation elongation factor activity; tRNA binding Biological Process: cellular protein metabolic process; translational elongation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; translation; gene expression
NCBI Summary:	This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]
UniProt Code:	P68104
NCBI GenInfo Identifier:	55584035
NCBI Gene ID:	1915
NCBI Accession:	P68104.1
UniProt Secondary Accession:	P68104,P04719, P04720, Q6IQ15,
UniProt Related Accession:	P68104
Molecular Weight:
NCBI Full Name:	Elongation factor 1-alpha 1
NCBI Synonym Full Names:	eukaryotic translation elongation factor 1 alpha 1
NCBI Official Symbol:	EEF1A1
NCBI Official Synonym Symbols:	CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
NCBI Protein Information:	elongation factor 1-alpha 1; EF-1-alpha-1; EF1a-like protein; CTCL tumor antigen; elongation factor Tu; cervical cancer suppressor 3; elongation factor 1 alpha subunit; prostate tumor-inducing protein 1; eukaryotic elongation factor 1 A-1; leukocyte receptor cluster member 7; leukocyte receptor cluster (LRC) member 7; translation elongation factor 1 alpha 1-like 14; glucocorticoid receptor AF-1 specific elongation factor; eukaryotic translation elongation factor 1 alpha 1-like 14
UniProt Protein Name:	Elongation factor 1-alpha 1
UniProt Synonym Protein Names:	Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1; Leukocyte receptor cluster member 7
UniProt Gene Name:	EEF1A1
UniProt Entry Name:	EF1A1_HUMAN

	Western blot analysis of extracts of various cell lines, using EEF1A1 antibody (CAB0974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of U2OS cells using EEF1A1 Rabbit pAb (CAB0974) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using EEF1A1 Rabbit pAb (CAB0974) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using EEF1A1 Rabbit pAb (CAB0974) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-EEF1A1 Antibody (CAB17857)	Human EEF1A1 / Elongation factor 1 alpha 1 ELISA Kit
Anti-eEF1A1 Antibody (CAB11545)	Human Elongation factor 1-alpha 1 (EEF1A1) ELISA Kit
Acetyl-EEF1A1 (K41) Antibody (PACO06060)
EEF1A1 Antibody (PACO08997)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)