The Actin (Plant Specific) Monoclonal Antibody (CAB23959) is a high-quality antibody developed for reliable detection and analysis of target proteins. Actin is an ancient conserved protein that is encoded by multiple isovariants in multi-cellular organisms. There are eight functional actin genes in the Arabidopsis genome. RRID Gene ID Swiss Prot Synonym ACT12; Actin(Plant Specific)
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Widely Range, Oryza sativa, Arabidopsis thaliana samples.
Product Name:
Actin (Plant Specific) Monoclonal Antibody
SKU:
CAB23959
Size:
100μL
Reactivity:
Widely Range, Oryza sativa, Arabidopsis thaliana
Clone Number:
ARC62861
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:10000 - 1:60000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACT12, Actin(Plant Specific)
Positive Sample:
Oryza sativa, Arabidopsis thaliana
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
45kDa
Observed MW:
45kDa
Actin is an ancient conserved protein that is encoded by multiple isovariants in multi-cellular organisms. There are eight functional actin genes in the Arabidopsis genome. RRID Gene ID Swiss Prot Synonym ACT12; Actin(Plant Specific)
Purification Method:
Affinity purification
Gene ID:
-
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Oryza sativa, using Actin(Plant Specific) Rabbit mAb (CAB23959) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of Arabidopsis thaliana, using Actin(Plant Specific) Rabbit mAb (CAB23959) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
