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Anti-ADA Antibody (CAB1019)

SKU:
CAB1019
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 1 Anti-ADA Antibody CAB1019
  • Cell Biology Antibodies 1 Anti-ADA Antibody CAB1019
€139 - €419
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Description

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Antibody Name:Anti-ADA Antibody
Antibody SKU:CAB1019
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-363 of human ADA (NP_000013.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, Jurkat, U-251MG, DU145, Mouse spleen, Mouse intestine, Mouse stomach
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-363 of human ADA (NP_000013.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAQT PAFD KPKV ELHV HLDG SIKP ETIL YYGR RRGI ALPA NTAE GLLN VIGM DKPL TLPD FLAK FDYY MPAI AGCR EAIK RIAY EFVE MKAK EGVV YVEV RYSP HLLA NSKV EPIP WNQA EGDL TPDE VVAL VGQG LQEG ERDF GVKA RSIL CCMR HQPN WSPK VVEL CKKY QQQT VVAI DLAG DETI PGSS LLPG HVQA YQEA VKSG IHRT VHAG EVGS AEVV KEAV DILK TERL GHGY HTLE DQAL YNRL RQEN MHFE ICPW SSYL TGAW KPDT EHAV IRLK NDQA NYSL NTDD PLIF KSTL DTDY QMTK RDMG FTEE EFKR LNIN AAKS SFLP EDEK RELL DLLY KAYG MPPS ASAG QNL
Gene ID:100
Uniprot:P00813
Cellular Location:Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle lumen, Extracellular side, Peripheral membrane protein
Calculated MW:40kDa
Observed MW:41kDa
Synonyms:ADA
Background:This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.
UniProt Protein Function:ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm.
UniProt Protein Details:

Protein type:Hydrolase; Nucleotide Metabolism - purine; EC 3.5.4.4

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cell surface; cytoplasm; cytosol; external side of plasma membrane; lysosome; membrane; plasma membrane

Molecular Function:adenosine deaminase activity; protein binding; zinc ion binding

Biological Process: adenosine catabolic process; hypoxanthine salvage; inosine biosynthetic process; negative regulation of adenosine receptor signaling pathway; purine nucleotide salvage; purine salvage; regulation of cell-cell adhesion mediated by integrin; response to hypoxia; T cell activation

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-negative, Due To Adenosine Deaminase Deficiency

NCBI Summary:This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]
UniProt Code:P00813
NCBI GenInfo Identifier:113339
NCBI Gene ID:100
NCBI Accession:P00813.3
UniProt Secondary Accession:P00813,Q53F92, Q6LA59,
UniProt Related Accession:P00813
Molecular Weight:40,764 Da
NCBI Full Name:Adenosine deaminase
NCBI Synonym Full Names:adenosine deaminase
NCBI Official Symbol:ADA  
NCBI Protein Information:adenosine deaminase
UniProt Protein Name:Adenosine deaminase
UniProt Synonym Protein Names:Adenosine aminohydrolase
Protein Family:ADAM
UniProt Gene Name:ADA  
UniProt Entry Name:ADA_HUMAN
Anti-ADA Antibody (CAB1019)Western blot analysis of extracts of various cell lines, using ADA antibody (CAB1019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-ADA Antibody (CAB1019)Immunofluorescence analysis of C6 cells using ADA antibody (CAB1019) at dilution of 1:100. Blue: DAPI for nuclear staining.

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