Anti-AFP Antibody (CAB11865)

Antibody Name:	Anti-AFP Mouse Monoclonal Antibody
Antibody SKU:	CAB11865
Antibody Size:	20uL, 50uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Mouse
Immunogen:	Recombinant protein of human AFP

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, human plasma, SH-SY5Y, mouse plasma, mouse liver, mouse kidney, rat plasma, rat liver

Immunogen:	Recombinant protein of human AFP
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	174
Uniprot:	P02771
Cellular Location:	Secreted
Calculated MW:	68kDa
Observed MW:	69kDa

Synonyms:

AFPD, FETA, HPAFP, AFP

Background:

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

UniProt Protein Function:	AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 4q13.3 Cellular Component: extracellular space; cytoplasm Molecular Function:metal ion binding Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of
NCBI Summary:	This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
UniProt Code:	P02771
NCBI GenInfo Identifier:	120042
NCBI Gene ID:	174
NCBI Accession:	P02771.1
UniProt Secondary Accession:	P02771,B2RBU3,
UniProt Related Accession:	P02771
Molecular Weight:	609
NCBI Full Name:	Alpha-fetoprotein
NCBI Synonym Full Names:	alpha-fetoprotein
NCBI Official Symbol:	AFP
NCBI Official Synonym Symbols:	AFPD; FETA; HPAFP
NCBI Protein Information:	alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein
UniProt Protein Name:	Alpha-fetoprotein
UniProt Synonym Protein Names:	Alpha-1-fetoprotein; Alpha-fetoglobulin
Protein Family:	AFP
UniProt Gene Name:	AFP
UniProt Entry Name:	FETA_HUMAN

	Western blot analysis of extracts of various cells, using AFP antibody (CAB11865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human liver cancer using AFP antibody (CAB11865) at dilution of 1:12.5 (40x lens).
	Immunofluorescence analysis of human liver cancer using AFP antibody (CAB11865) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-AFP Antibody (CAB0200)	Human alphaFP (Alpha-Fetoprotein) CLIA Kit (HUES00068)
Anti-AFP Antibody (CAB11013)	Human alphaFP (Alpha-Fetoprotein) ELISA Kit (HUES01357)
Anti-AFP Antibody (CAB16750)	Human Alpha-fetoprotein (AFP) ELISA Kit
Anti-AFP Antibody (CAB17457)
Anti-AFP Mouse Monoclonal Antibody (CAB17898)

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)