Anti-AFP Antibody (CAB11013)

Antibody Name:	Anti-AFP Antibody
Antibody SKU:	CAB11013
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 360-609 of human AFP (NP_001125.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 360-609 of human AFP (NP_001125.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RRHP QLAV SVIL RVAK GYQE LLEK CFQT ENPL ECQD KGEE ELQK YIQE SQAL AKRS CGLF QKLG EYYL QNAF LVAY TKKA PQLT SSEL MAIT RKMA ATAA TCCQ LSED KLLA CGEG AADI IIGH LCIR HEMT PVNP GVGQ CCTS SYAN RRPC FSSL VVDE TYVP PAFS DDKF IFHK DLCQ AQGV ALQT MKQE FLIN LVKQ KPQI TEEQ LEAV IADF SGLL EKCC QGQE QEVC FAEE GQKL ISKT RAAL GV
Gene ID:	174
Uniprot:	P02771
Cellular Location:	Secreted
Calculated MW:	68kDa
Observed MW:	68kDa

Synonyms:

AFPD, FETA, HPAFP, AFP

Background:

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

UniProt Protein Function:	AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 4q13.3 Cellular Component: extracellular space; cytoplasm Molecular Function:metal ion binding Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of
NCBI Summary:	This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
UniProt Code:	P02771
NCBI GenInfo Identifier:	120042
NCBI Gene ID:	174
NCBI Accession:	P02771.1
UniProt Secondary Accession:	P02771,B2RBU3,
UniProt Related Accession:	P02771
Molecular Weight:	609
NCBI Full Name:	Alpha-fetoprotein
NCBI Synonym Full Names:	alpha-fetoprotein
NCBI Official Symbol:	AFP
NCBI Official Synonym Symbols:	AFPD; FETA; HPAFP
NCBI Protein Information:	alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein
UniProt Protein Name:	Alpha-fetoprotein
UniProt Synonym Protein Names:	Alpha-1-fetoprotein; Alpha-fetoglobulin
Protein Family:	AFP
UniProt Gene Name:	AFP
UniProt Entry Name:	FETA_HUMAN

	Western blot analysis of extracts of mouse liver, using AFP antibody (CAB11013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunofluorescence analysis of human liver cancer using AFP antibody (CAB11013) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HepG2 cells (upper left) and LO2 cells (negative sample control) (upper right) use AFP Rabbit pAb (red, A11013) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-AFP Antibody (CAB0200)	Human alphaFP (Alpha-Fetoprotein) CLIA Kit (HUES00068)
Anti-AFP Mouse Monoclonal Antibody (CAB11865)	Human alphaFP (Alpha-Fetoprotein) ELISA Kit (HUES01357)
Anti-AFP Antibody (CAB16750)	Human Alpha-fetoprotein (AFP) ELISA Kit
Anti-AFP Antibody (CAB17457)
Anti-AFP Mouse Monoclonal Antibody (CAB17898)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)