Anti-AGXT Antibody (CAB8397)

Antibody Name:	Anti-AGXT Antibody
Antibody SKU:	CAB8397
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 183-392 of human AGXT (NP_000021.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human
Positive Samples:	LO2, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 183-392 of human AGXT (NP_000021.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DSVA SLGG TPLY MDRQ GIDI LYSG SQKA LNAP PGTS LISF SDKA KKKM YSRK TKPF SFYL DIKW LANF WGCD DQPR MYHH TIPV ISLY SLRE SLAL IAEQ GLEN SWRQ HREA AAYL HGRL QALG LQLF VKDP ALRL PTVT TVAV PAGY DWRD IVSY VIDH FDIE IMGG LGPS TGKV LRIG LLGC NATR ENVD RVTE ALRA ALQH CPKK KL
Gene ID:	189
Uniprot:	P21549
Cellular Location:	Mitochondrion, Peroxisome
Calculated MW:	43kDa
Observed MW:	43kDa

Synonyms:	AGXT, AGT, AGT1, AGXT1, PH1, SPAT, SPT, TLH6
Background:	This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria.

UniProt Protein Function:	AGXT: Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - glycine, serine and threonine; EC 2.6.1.51; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Motility/polarity/chemotaxis; EC 2.6.1.44 Chromosomal Location of Human Ortholog: 2q37.3 Cellular Component: peroxisomal matrix; peroxisome Molecular Function:alanine-glyoxylate transaminase activity; amino acid binding; protein binding; protein homodimerization activity; protein self-association; pyridoxal phosphate binding; receptor binding; serine-pyruvate transaminase activity; transaminase activity Biological Process: glycine biosynthetic process, by transamination of glyoxylate; glyoxylate catabolic process; glyoxylate metabolic process; L-alanine catabolic process; L-cysteine catabolic process; proteasomal protein catabolic process Disease: Hyperoxaluria, Primary, Type I
NCBI Summary:	This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]
UniProt Code:	P21549
NCBI GenInfo Identifier:	134855
NCBI Gene ID:	189
NCBI Accession:	P21549.1
UniProt Secondary Accession:	P21549,Q53QU6,
UniProt Related Accession:	P21549
Molecular Weight:	43010 Da
NCBI Full Name:	Serine--pyruvate aminotransferase
NCBI Synonym Full Names:	alanine-glyoxylate aminotransferase
NCBI Official Symbol:	AGXT
NCBI Official Synonym Symbols:	AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1
NCBI Protein Information:	serine--pyruvate aminotransferase
UniProt Protein Name:	Serine--pyruvate aminotransferase
UniProt Synonym Protein Names:	Alanine--glyoxylate aminotransferase (EC:2.6.1.44); AGT
Protein Family:	Serine--pyruvate aminotransferase
UniProt Gene Name:	AGXT
UniProt Entry Name:	SPYA_HUMAN

	Western blot analysis of extracts of various cell lines, using AGXT antibody (CAB8397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human liver using AGXT Rabbit pAb (CAB8397) at dilution of 1:150 (40x lens).

Antibodies

Anti-AGXT Antibody (CAB13943)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)