The AHSP Antibody (CAB6465) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting AHSP in various sample types, making it ideal for Western blot applications.AHSP, or alpha-hemoglobin-stabilizing protein, plays a crucial role in stabilizing alpha-globin chains and preventing their degradation, ensuring proper hemoglobin formation and function. Dysregulation of AHSP has been linked to various blood disorders, including thalassemia and anemia, making it a valuable target for research in hematology and transfusion medicine.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
AHSP Antibody
SKU:
CAB6465
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EDRF, ERAF, AHSP
Positive Sample:
Mouse liver, Rat liver
Cellular Localization:
Cytoplasm.
Calculated MW:
12kDa
Observed MW:
12kDa
This gene encodes a molecular chaperone which binds specifically to free alpha-globin and is involved in hemoglobin assembly. The encoded protein binds to monomeric alpha-globin until it has been transferred to beta-globin to form a heterodimer, which in turn binds to another heterodimer to form the stable tetrameric hemoglobin. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
51327
RRID
AB_2767067
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using AHSP Rabbit pAb (CAB6465) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
