Anti-AK4 Antibody (CAB13461)

Antibody Name:	Anti-AK4 Antibody
Antibody SKU:	CAB13461
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human AK4 (NP_037542.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HepG2, Raji, HeLa, SW480, Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human AK4 (NP_037542.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASK LLRA VILG PPGS GKGT VCQR IAQN FGLQ HLSS GHFL RENI KAST EVGE MAKQ YIEK SLLV PDHV ITRL MMSE LENR RGQH WLLD GFPR TLGQ AEAL DKIC EVDL VISL NIPF ETLK DRLS RRWI HPPS GRVY NLDF NPPH VHGI DDVT GEPL VQQE DDKP EAVA ARLR QYKD VAKP VIEL YKSR GVLH QFSG TETN KIWP YVYT LFSN KITP IQSK EAY
Gene ID:	205
Uniprot:	P27144
Cellular Location:	Mitochondrion matrix
Calculated MW:	25kDa
Observed MW:	25kDa

Synonyms:

AK4, AK 4, AK3, AK3L1, AK3L2

Background:

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1.

UniProt Protein Function:	AK3L1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. May also be active with GTP. Belongs to the adenylate kinase family.
UniProt Protein Details:	Protein type:Nucleotide Metabolism - purine; EC 2.7.4.10; EC 2.7.4.6; Mitochondrial; Kinase, other Chromosomal Location of Human Ortholog: 1p31.3 Cellular Component: mitochondrion; mitochondrial matrix Molecular Function:GTP binding; nucleoside diphosphate kinase activity; adenylate kinase activity; nucleoside triphosphate adenylate kinase activity; ATP binding Biological Process: response to drug; ATP metabolic process; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; ADP biosynthetic process; brain development; liver development; AMP metabolic process; GTP metabolic process
NCBI Summary:	This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]
UniProt Code:	P27144
NCBI GenInfo Identifier:	125157
NCBI Gene ID:	205
NCBI Accession:	P27144.1
UniProt Related Accession:	P27144
Molecular Weight:	~ 25kDa
NCBI Full Name:	Adenylate kinase 4, mitochondrial
NCBI Synonym Full Names:	adenylate kinase 4
NCBI Official Symbol:	AK4
NCBI Official Synonym Symbols:	AK3; AK 4; AK3L1; AK3L2
NCBI Protein Information:	adenylate kinase 4, mitochondrial
UniProt Protein Name:	Adenylate kinase 4, mitochondrial
UniProt Synonym Protein Names:	Adenylate kinase 3-like; GTP:AMP phosphotransferase AK4
UniProt Gene Name:	AK4
UniProt Entry Name:	KAD4_HUMAN

	Western blot analysis of extracts of various cell lines, using AK4 antibody (CAB13461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of A549 cells using AK4 antibody (CAB13461). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug AK4 antibody (CAB13461). Western blot was performed from the immunoprecipitate using AK4 antibody (CAB13461) at a dilition of 1:1000.

Antibodies

Anti-AK4 Antibody (CAB2050)

Anti-AK4 Antibody (CAB19854)[KO Validated]

Anti-AK4 Antibody (CAB19855)[KO Validated]

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)