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Anti-AKAP8 Antibody (CAB3049)

SKU:
CAB3049
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • Immunology Antibodies 2 Anti-AKAP8 Antibody CAB3049
  • Immunology Antibodies 2 Anti-AKAP8 Antibody CAB3049
€139 - €419
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Description

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Antibody Name:Anti-AKAP8 Antibody
Antibody SKU:CAB3049
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 480-692 of human AKAP8 (NP_005849.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:LO2, 293T, BxPC-3, HeLa, Jurkat, mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 480-692 of human AKAP8 (NP_005849.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HCLA CDML IPAQ PQLL QRHL HSVD HNHN RRLA AEQF KKTS LHVA KSVL NNRH IVKM LEKY LKGE DPFT SETV DPEM EGDD NLGG EDKK ETPE EVAA DVLA EVIT AAVR AVDG EGAP APES SGEP AEDE GPTD TAEA GSDP QAEQ LLEE QVPC GTAH EKGV PKAR SEAA EAGN GAET MAAE AESA QTRV APAP AAAD AEVE QTDA ESKD AVPT E
Gene ID:10270
Uniprot:O43823
Cellular Location:Nucleus matrix
Calculated MW:76kDa
Observed MW:110kDa
Synonyms:AKAP8, AKAP 95, AKAP-8, AKAP-95, AKAP95
Background:This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9.
UniProt Protein Function:AKAP8: Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Belongs to the AKAP95 family.
UniProt Protein Details:

Protein type:Cell cycle regulation; RNA-binding; Mitochondrial; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleoplasm; Golgi apparatus; nuclear matrix; membrane; mitochondrion; condensed chromosome; female pronucleus; nucleus

Molecular Function:zinc ion binding; double-stranded DNA binding

Biological Process: mitosis; mitotic chromosome condensation; signal transduction

NCBI Summary:This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]
UniProt Code:O43823
NCBI GenInfo Identifier:5902752
NCBI Gene ID:10270
NCBI Accession:O43823.1
UniProt Related Accession:O43823
Molecular Weight:692
NCBI Full Name:A-kinase anchor protein 8
NCBI Synonym Full Names:A kinase (PRKA) anchor protein 8
NCBI Official Symbol:AKAP8  
NCBI Official Synonym Symbols:AKAP-8; AKAP95; AKAP 95; AKAP-95  
NCBI Protein Information:A-kinase anchor protein 8; A-kinase anchor protein, 95kDa
UniProt Protein Name:A-kinase anchor protein 8
UniProt Synonym Protein Names:A-kinase anchor protein 95 kDa; AKAP 95
Protein Family:A-kinase anchor protein
UniProt Gene Name:AKAP8  
UniProt Entry Name:AKAP8_HUMAN
Anti-AKAP8 Antibody (CAB3049)Western blot analysis of extracts of various cell lines, using AKAP8 antibody (CAB3049) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-AKAP8 Antibody (CAB3049)Confocal immunofluorescence analysis of U2OS cells using AKAP8 Polyclonal Antibody (CAB3049) at dilution of 1:200. Blue: DAPI for nuclear staining.

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