The Aldosterone ELISA Kit is specifically designed for the precise measurement of aldosterone levels in serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a variety of research applications.Aldosterone is a vital hormone that regulates electrolyte balance and blood pressure by acting on the kidneys to retain sodium and water while excreting potassium. Imbalances in aldosterone levels can lead to conditions such as hypertension, heart failure, and kidney disease, making it a crucial biomarker for studying these diseases and evaluating treatment effectiveness.Overall, the Aldosterone ELISA Kit offers researchers a valuable tool for accurately quantifying aldosterone levels, contributing to a better understanding of its role in health and disease.
Product Name:
ALD (Aldosterone) ELISA Kit
SKU:
UNES00045
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
