Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3F8 (STMA00193). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody (STMA00193) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT.
Alpha Synuclein Antibody [3F8] is a mouse monoclonal antibody directed against Alpha Synuclein, offered by Assay Genie for research applications. Reported applications include WB, DB, IHC, ICC/IF and ELISA. Reported reactivity: Human, Mouse and Rat. Supplied as IgG1, purified by Protein G.
Alpha-synuclein (SNCA) is a neuronal protein predominantly expressed in the brain, where it is highly concentrated at presynaptic nerve terminals, suggesting a key role in synaptic transmission and plasticity (1). In addition to its synaptic localization, alpha-synuclein is abundantly expressed in mitochondria-rich brain regions, including the olfactory bulb, hippocampus, striatum, and thalamus, implicating it in mitochondrial function and energy metabolism (2). Functionally, alpha-synuclein has been shown to interact significantly with tubulin (3), indicating a potential role as a microtubule-associated protein involved in cytoskeletal organization and intracellular transport. This interaction may be critical for maintaining neuronal structure and function, particularly in long-range axonal projections. Importantly, alpha-synuclein is essential for normal cognitive development. Genetic or functional inactivation of SNCA has been associated with impairments in spatial learning and working memory, underscoring its role in higher-order brain functions (4). Given its physiological importance and pathological aggregation in disorders such as Parkinson’s disease and dementia with Lewy bodies, alpha-synuclein remains a focal point in neurodegenerative disease research. Understanding its normal function and mechanisms of misfolding is vital for developing targeted diagnostics and therapeutics. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, DB, IHC, ICC/IF, ELISA
Antibody Isotype:
IgG1
Purification:
Protein G Purified
Concentration:
1 mg/ml
Reactivity:
Human, Mouse, Rat
Recommended Dilution:
WB (1:1000); DB (1:1000); IHC (1:200); ICC/IF (1:100); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Specificity:
Binds within the 101-140 aa region of human alpha synuclein, determined using WB and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to Alpha synuclein. Does not detect truncated alpha Synuclein (aa 1-114)
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
Alternative Names:
Alpha Synuclein, α-Synuclein, SNCA, Snca, SYN, alphaSYN, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease familial 1, PARK1, PARK 1, PARK4, PARK 4, Parkinson disease (autosomal dominant, Lewy body) 4
References:
1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.
