AMN Antibody (PACO59445)
- SKU:
- PACO59445
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | AMN Antibody (PACO59445) |
| Antibody SKU: | PACO59445 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Protein amnionless protein (25-163AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO59445 diluted at 1:600 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO59445 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Necessary for efficient absorption of vitamin B12. Required for normal CUBN-mediated protein transport in the kidney. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm (By similarity). |
| Synonyms: | Protein amnionless, AMN |
| UniProt Protein Function: | Necessary for efficient absorption of vitamin B12 (PubMed:12590260, PubMed:14576052). Required for normal CUBN-mediated protein transport in the kidney. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm (). |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene is a type I transmembrane protein. It is thought to modulate bone morphogenetic protein (BMP) receptor function by serving as an accessory or coreceptor, and thus facilitates or hinders BMP binding. It is known that the mouse AMN gene is expressed in the extraembryonic visceral endoderm layer during gastrulation, but it is found to be mutated in amnionless mouse. The encoded protein has sequence similarity to short gastrulation (Sog) and procollagen IIA proteins in Drosophila. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9BXJ7 |
| NCBI GenInfo Identifier: | 37222204 |
| NCBI Gene ID: | 81693 |
| NCBI Accession: | AAQ89949.1 |
| UniProt Secondary Accession: | Q9BXJ7,Q6UX83 |
| UniProt Related Accession: | Q9BXJ7 |
| Molecular Weight: | 47,754 Da |
| NCBI Full Name: | AMN, partial |
| NCBI Synonym Full Names: | amnion associated transmembrane protein |
| NCBI Official Symbol: | AMNÂ Â |
| NCBI Official Synonym Symbols: | PRO1028; amnionless  |
| NCBI Protein Information: | protein amnionless |
| UniProt Protein Name: | Protein amnionless |
| UniProt Synonym Protein Names: | |
| Protein Family: | AMP nucleosidase |
| UniProt Gene Name: | AMNÂ Â |
| UniProt Entry Name: |