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AMO (Amoxicillin) ELISA Kit (FSES0049)

SKU:
FSES0049
Product Type:
ELISA Kit
Applications:
ELISA
Research Area:
Food Science
€599
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Description

Technical Manual

AMO (Amoxicillin) ELISA Kit

Kit Name: AMO (Amoxicillin) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Amoxicillin (AMO) in samples, such as muscle, eggs, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, AMO in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AMO antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of AMO. The concentration of AMO in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.03 ppb (ng/mL)
Reaction Mode: 25°C, 30 min~30min~20min
Detection Limit: Milk---0.2 ppb; Muscle, Eggs ---1 ppb; Duck---1.5 ppb
Cross Reactivity:

Amoxicillin

100%

Penicillin

16%

Ampicillin

9%

Nafcillin

2.4%

Oxacillin

7.1%

Cloxacillin

6.3%

Dicloxacillin

11.8%

Azlocillin

14.7%

Methicillin

5.2%

Sample Recovery Rate: 90%±30%.
Kits Components:
Item Specifications
ELISA Microtiter plate 96 wells
Standard Liquid 1 mL each (0 ppb, 0.03 ppb, 0.09 ppb, 0.27 ppb, 0.81 ppb, 2.43 ppb)
Antibody Working Solution 7 mL
HRP Conjugate 12 mL
5×Concentrated Sample Solution 1 40 mL
50×Concentrated Sample Solution 2 10 mL
Substrate Reagent A 6 mL
Substrate Reagent B 6 mL
Stop Solution 6 mL
20×Concentrated Wash Buffer 25 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate reader, Printer, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01g),
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: ZnSO4·7H2O.
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.