Anti-ANO2 Antibody (CAB13109)

Antibody Name:	Anti-ANO2 Antibody
Antibody SKU:	CAB13109
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ANO2 (NP_001265526.1).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ANO2 (NP_001265526.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPED IPLL PGSP RRLS PQAG SRGG QGPK HGQQ CLKM PGPR APGL QGGS NRDP GQPC GGES TRSS SVIN NYLD ANEP VSLE ARLS RMHF HDSQ RKVD YVLA YHYR KRGV HLAQ GFPG HSLA IVSN GETG KEPH AGGP GDIE LGPL DALE EERK EQRE EFEH NLME AGLE LEKD LENK SQGS IFVR IHAP WQVL AREA EFLK
Gene ID:	57101
Uniprot:	Q9NQ90
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	113kDa
Observed MW:	114kDa

Synonyms:	ANO2, C12orf3, TMEM16B
Background:

UniProt Protein Function:	ANO2: Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transporter, ion channel; Transporter; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 12p13.3 Cellular Component: nucleoplasm; nonmotile primary cilium; plasma membrane Molecular Function:protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity Biological Process: transmembrane transport
NCBI Summary:	ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]).[supplied by OMIM, Jan 2011]
UniProt Code:	Q9NQ90
NCBI GenInfo Identifier:	262527528
NCBI Gene ID:	57101
NCBI Accession:	Q9NQ90.2
UniProt Secondary Accession:	Q9NQ90,Q9H847, C4N787,
UniProt Related Accession:	Q9NQ90
Molecular Weight:	1003
NCBI Full Name:	Anoctamin-2
NCBI Synonym Full Names:	anoctamin 2, calcium activated chloride channel
NCBI Official Symbol:	ANO2
NCBI Official Synonym Symbols:	C12orf3; TMEM16B
NCBI Protein Information:	anoctamin-2; transmembrane protein 16B (eight membrane-spanning domains)
UniProt Protein Name:	Anoctamin-2
UniProt Synonym Protein Names:	Transmembrane protein 16B
Protein Family:	Anoctamin
UniProt Gene Name:	ANO2
UniProt Entry Name:	ANO2_HUMAN

	Western blot analysis of extracts of HeLa cells, using ANO2 antibody (CAB13109) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded rat retina using ANO2 antibody (CAB13109) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse retina using ANO2 antibody (CAB13109) at dilution of 1:200 (40x lens).

Antibodies

Anti-ANO2 Antibody (CAB3684)

ANO2 Antibody (PACO07741)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)