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Cell Biology Antibodies 17 Anti-alpha 1 Spectrin Antibody CAB9597
Key Features

alpha 1 Spectrin Rabbit Monoclonal Antibody (CAB9597)

Datasheet MSDS
SKU CAB9597
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms EL2, HPP, HS3, SPH3, SPTA, alpha 1 Spectrin
Research Use Cell Biology
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

alpha 1 Spectrin Monoclonal Antibody (CAB9597)

The alpha 1 Spectrin Monoclonal Antibody (CAB9597) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting.Alpha-1 spectrin plays a critical role in maintaining cell shape, supporting cell function, and facilitating intracellular transport. Dysregulation of alpha-1 spectrin has been implicated in various diseases, including hereditary spherocytosis and certain types of cancer.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:alpha 1 Spectrin Monoclonal Antibody
SKU:CAB9597
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC1650
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:LRGL NYYL PMVE EDEH EPKF EKFL DAVD PGRK GYVS LEDY TAFL IDKE SENI KSSD EIEN AFQA LAEG KSYI TKED MKQA LTPE QVSF CATH MQQY MDPR G
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:EL2, HPP, HS3, SPH3, SPTA, alpha 1 Spectrin
Positive Sample:K-562, Mouse lung, Rat heart
Cellular Localization:Cytoplasm, Cell Cortex, Cytoskeleton.
Calculated MW:280kDa
Observed MW:280kDa

This gene encodes a member of a family of molecular scaffold proteins that link the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms a component of the erythrocyte plasma membrane. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3.

Purification MethodAffinity purification
Gene ID6708
RRIDAB_2863731
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using alpha 1 Spectrin Rabbit mAb (CAB9597) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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