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Anti-Bcl 2 Mouse Antibody (CAB20777)

SKU:
CAB20777
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Goat
Isotype:
Horseradish peroxidase conjugated IgG
  • Immunofluorescence analysis of PC-12 cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Bcl-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
€179 - €469
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Description

Antibody Name:Anti-Bcl 2 Mouse Antibody (CAB20777)
Antibody SKU:CAB20777
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Mouse
Immunogen:Recombinant protein of mouse Bcl-2.
Application:Western blotting, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Raji, Jurkat, 293T, NIH/3T3
Immunogen:Recombinant protein of mouse Bcl-2.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein
Calculated MW:22kDa/26kDa
Observed MW:26KDa
Synonyms:Bcl-2, PPP1R50, BCL2
Background:This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Immunofluorescence analysis of PC-12 cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of PC-12 cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of HeLa cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Bcl-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Western blot analysis of extracts of various cell lines, using Bcl-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

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