Anti-Beta Actin Mouse Monoclonal Antibody (CABC004)

SKU:
CABC004
  • Loading Controls Anti-Beta Actin Mouse Monoclonal Antibody CABC004
  • Loading Controls Anti-Beta Actin Mouse Monoclonal Antibody CABC004
  • Loading Controls Anti-Beta Actin Mouse Monoclonal Antibody CABC004
  • Loading Controls Anti-Beta Actin Mouse Monoclonal Antibody CABC004
  • Loading Controls Anti-Beta Actin Mouse Monoclonal Antibody CABC004
€179

Description

Technical Manual

Product Name: Anti-Beta Actin Mouse Monoclonal Antibody
Description: Mouse monoclonal to beta Actin
Host Species: Mouse
Species Reactivity: Human, Mouse, Rat
Immunogen: Fusion protein of human Beta-actin
Tested Applications: WB, IHC, IF, IP
Positive Samples: HeLa, TE-1, 293T, NIH/3T3, Mouse brain, Mouse testis, C6, Rat brain, Rat lung
Synonyms: ACTB, BRWS1, PS1TP5BP1, beta actin, actin
Size: 100 µL
Storage Instructions: Store at -20°C. Avoid freeze / thaw cycles.
Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Dilution
WB 1:10000 - 1:100000
Calculated MW: 41kDa
Observed MW: 42kDa
IHC 1:100- 1:200
IF 1:50- 1:200
IP 1:50 - 1:200

Anti-Beta Actin Mouse Monoclonal Antibody (CABC004)

Western blot analysis of extracts of various cell lines, using b-actin antibody (CABC004) at 1:80000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Anti-Beta Actin Mouse Monoclonal Antibody (CABC004)

Western blot analysis of extracts of NIH/3T3 cells, using beta-actin antibody (CABC004) at 1:10000-1:2560000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Anti-Beta Actin Mouse Monoclonal Antibody (CABC004)

Immunohistochemistry of paraffin-embedded rat spleen using beta-actin antibody (CABC004) at dilution of 1:500 (40x lens).

Anti-Beta Actin Mouse Monoclonal Antibody (CABC004)

Immunohistochemistry of paraffin-embedded human stomach using Beta-actin antibody (CABC004) at dilution of 1:500 (40x lens).

Anti-Beta Actin Mouse Monoclonal Antibody (CABC004)

Immunohistochemistry of paraffin-embedded mouse spleen using Beta-actin antibody (CABC004) at dilution of 1:500 (40x lens).
UniProt Protein Function: Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
UniProt Protein Details:

Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Ref.11 Ref.12 Ref.15 Ref.22

Subcellular location: Cytoplasm ۼ cytoskeleton. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Ref.17

Post-translational modification: ISGylated. Ref.13Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced

Involvement in Disease: Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [

MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. Ref.23Defects in ACTB are the cause of Baraitser-Winter syndrome type 1 (BRWS1) [

MIM:243310]. A rare developmental disorder characterized by the combination of congenital ptosis, high-arched eyebrows, hypertelorism, ocular colobomata, and a brain malformation consisting of anterior-predominant lissencephaly. Other typical features include postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss. Ref.25

Miscellaneous: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Sequence similarities: Belongs to the actin family.

NCBI Summary: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]
UniProt Code: P60709
NCBI GenInfo Identifier: 46397333
NCBI Gene ID: 60
NCBI Accession: P60709.1
UniProt Secondary Accession: P60709,P02570, P70514, P99021, Q11211, Q64316, Q75MN2 Q96B34, Q96HG5,
UniProt Related Accession: P60709
Molecular Weight: 67 KD
NCBI Full Name: Actin, cytoplasmic 1
NCBI Synonym Full Names: actin, beta
NCBI Official Symbol: ACTB
NCBI Official Synonym Symbols: BRWS1; PS1TP5BP1
NCBI Protein Information: actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
UniProt Protein Name: Actin, cytoplasmic 1
UniProt Synonym Protein Names: Beta-actin
Protein Family: Actin
UniProt Gene Name: ACTB
UniProt Entry Name: ACTB_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Mouse
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
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