Western blot analysis of various lysates, using CD166/ALCAM Rabbit mAb (CAB23441) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The CD166/ALCAM Monoclonal Antibody (CAB23441) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated through monoclonal antibody technology, is highly specific and sensitive for detecting CD166 in human samples, enabling precise analysis in various research settings.CD166, also known as ALCAM, plays a crucial role in cell-cell adhesion and communication, particularly in the immune system and cancer progression. As a key player in cell adhesion and migration, CD166 is implicated in processes such as T cell activation, lymphocyte trafficking, and tumor metastasis. The CD166 Monoclonal Antibody allows for accurate detection and characterization of CD166 expression, making it ideal for studies in immunology, cancer biology, and cell biology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD166/ALCAM Monoclonal Antibody
SKU:
CAB23441
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC57940
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:2500 - 1:25000
IF/ICC
1:400 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MEMD, CD166, CD166/ALCAM
Positive Sample:
HeLa, HT-1080, SH-SY5Y, Mouse lung, Mouse brain, Rat lung
Cellular Localization:
Membrane, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
65kDa
Observed MW:
100-105kDa
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
Purification Method
Affinity purification
Gene ID
214
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CD166/ALCAM Rabbit mAb (CAB23441) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): K-562. Exposure time: 60s.
Confocal imaging of SH-SY5Y cells using CD166/ALCAM Rabbit mAb (CAB23441, dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
