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Immunohistochemistry of paraffin-embedded rat brain using eIF1A Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Key Features

eIF1A Monoclonal Antibody (CAB20954)

Datasheet MSDS
SKU CAB20954
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse
Host Species Rabbit
Synonyms EIF1A, EIF4C, eIF-1A, eIF-4C, EIF1AP1, eIF1A
$173 - $603

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Description

system_update_altDatasheetsystem_update_altMSDS

eIF1A Monoclonal Antibody (CAB20954)

The eIF1A Monoclonal Antibody (CAB20954) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to EIF1A, researchers can detect and analyze this protein in various cell types, making it an ideal tool for studies in molecular biology and cancer research.EIF1A plays a crucial role in the translation initiation process, promoting the binding of the initiator tRNA to the small ribosomal subunit.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:eIF1A Monoclonal Antibody
SKU:CAB20954
Size:20μL, 100μL
Reactivity:Human, Mouse
Clone Number:ARC2941
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:LRKK VWIN TSDI ILVG LRDY QDNK ADVI LKYN ADEA RSLK AYGE LPEH AKIN ETDT FGPG DDDE IQFD DIGD DDED IDDI
Tested Applications:WB ELISA
Recommended Dilution:
WB1:1000 - 1:5000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:EIF1A, EIF4C, eIF-1A, eIF-4C, EIF1AP1, eIF1A
Positive Sample:HeLa, C2C12
Cellular Localization:Cytoplasm, Cytosol, Synapse.
Calculated MW:16kDa
Observed MW:17kDa

This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA.

Purification MethodAffinity purification
Gene ID1964
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using eIF1A Rabbit mAb (CAB20954) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5 s.
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