Western blot analysis of extracts of various cell lines, using Glyt2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
The Glyt2 Monoclonal Antibody (CAB20931) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is optimized for use in various applications such as Western blot and immunohistochemistry.GLYT2 is known to be essential for maintaining the balance of neurotransmitters in the central nervous system, making it a promising target for investigating neurological disorders and developing potential therapeutics. By targeting the GLYT2 protein, researchers can gain insights into its function and potential implications in conditions such as epilepsy, schizophrenia, and neuropathic pain.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Glyt2 Monoclonal Antibody
SKU:
CAB20931
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2912
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NET1, GLYT2, HKPX3, GLYT-2, Glyt2
Positive Sample:
U-937, SH-SY5Y, Mouse spinal cord, Mouse brain, Mouse lung, Rat spinal cord, Rat brain
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
87kDa
Observed MW:
87kDa
This gene encodes a sodium- and chloride-dependent glycine neurotransmitter transporter. This integral membrane glycoprotein is responsible for the clearance of extracellular glycine during glycine-mediated neurotransmission. This protein is found in glycinergic axons and maintains a high presynaptic pool of neurotransmitter at glycinergic synapses. Mutations in this gene cause hyperekplexia; a heterogenous neurological disorder characterized by exaggerated startle responses and neonatal apnea. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
9152
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Glyt2 Rabbit mAb (CAB20931) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
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