The HCoV-NL63 Spike S2 Antibody (CAB20611) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised against the Spike S2 protein of the virus, is highly specific and reactive with human samples. It is validated for use in various applications, including immunofluorescence and ELISA.The Spike S2 protein is an important component of the HCoV-NL63 virus, involved in viral entry and infectivity. By targeting this protein with the CAB20611 antibody, researchers can detect and study the virus in infected cells, tissues, and patient samples.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against HCoV-NL63 samples.
Product Name:
HCoV-NL63 Spike S2 Antibody
SKU:
CAB20611
Size:
20μL, 100μL
Reactivity:
HCoV-NL63
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
Human coronavirus (HCoV-NL63) Spike Protein (S1+S2 ECDHis Tag)
Calculated MW:
150kDa
Observed MW:
160kDa
S1 region attaches the virion to the cell membrane by interacting with host ACE2, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Purification Method
Affinity purification
Gene ID
2943499
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Human coronavirus (HCoV-NL63) Spike Protein (S1+S2 ECDHis Tag), using HCoV-NL63 Spike S2 Rabbit pAb (CAB20611) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
