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Anti-HLA-DRB4 Antibody (CAB0439)

SKU:
CAB0439
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
HLA-DRB4
DR4
DRB4
HLA-DR4B
major histocompatibility complex
class II
DR beta 4
€139 - €419
Frequently bought together:

Description

Product Name:HLA-DRB4 Rabbit mAb
Product Code:CAB0439
Size:20uL, 50uL, 100uL
Synonyms:HLA-DRB4, DR4, DRB4, HLA-DR4B, major histocompatibility complex, class II, DR beta 4
Applications:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human HLA-DRB4.
Applications:WB
Recommended Dilutions:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:Raji
Immunogen:A synthesized peptide derived from human HLA-DRB4.
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3126
Uniprot:P13762
Cellular Location:Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane
Calculated MW:30kDa
Observed MW:30KDa
UniProt Protein Function:HLA-DRB4 iso2: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; late endosome membrane; lysosomal membrane; plasma membrane; trans-Golgi network membrane

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; T cell costimulation; T cell receptor signaling pathway

NCBI Summary:HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008]
UniProt Code:P13762
NCBI GenInfo Identifier:281371554
NCBI Gene ID:3126
NCBI Accession:P13762.2
UniProt Secondary Accession:P13762,O78042, P79664, Q29889, Q30163, Q6TLK6, Q860N4 Q861F3, Q8WLT9, Q9BS54, B0S863,
UniProt Related Accession:P13760,P13762
Molecular Weight:29,941 Da
NCBI Full Name:HLA class II histocompatibility antigen, DR beta 4 chain
NCBI Synonym Full Names:major histocompatibility complex, class II, DR beta 4
NCBI Official Symbol:HLA-DRB4  
NCBI Official Synonym Symbols:DR4; DR-4; DRB4; HLA-DR4B  
NCBI Protein Information:major histocompatibility complex, class II, DR beta 4
UniProt Protein Name:HLA class II histocompatibility antigen, DR beta 4 chain
UniProt Synonym Protein Names:MHC class II antigen DRB4
Protein Family:HLA class II histocompatibility antigen
UniProt Gene Name:HLA-DRB4  
UniProt Entry Name:DRB4_HUMAN

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