Anti-Human CD16 [3G8] In Vivo Antibody

Product Name:	Anti-Human CD16 [3G8] In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0112
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	3G8
Protein:	CD16
Product Type:	Monoclonal Antibody
Synonyms:	Fc-gamma RIII, Fc Gamma RIII
Isotype:	Mouse IgG1 κ
Reactivity:	Baboon, Chimpanzee, Cynomolgus Monkey, Marmoset, Pigtailed Macaque, Rhesus Monkey, Squirrel Monkey, Human
Immunogen:	Human PMN cells
Applications:	B, CyTOF®, FC, IHC FF, IP
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0112	Anti-Human CD16 [3G8] In Vivo Antibody - Low Endotoxin	Low
IVMB0113	Anti-Human CD16 [3G8] In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	B, CyTOF®, FC, IHC FF, IP
Recommended Usage:	FC The suggested concentration for this 3G8 antibody for staining cells in flow cytometry is ≤ 2.0 µg per 10⁶ in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:	Baboon, Chimpanzee, Cynomolgus Monkey, Marmoset, Pigtailed Macaque, Rhesus Monkey, Squirrel Monkey, Human

Host Species:	Mouse
Specificity:	Clone 3G8 recognizes an epitope on human CD16. This clone also cross-reacts with non-human primate CD16.
Antigen Distribution:	CD16 is expressed on NK cells, activated monocytes, macrophages, neutrophils, and placental trophoblasts.
Immunogen:	Human PMN cells
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD16 antibody, 3G8, recognizes human CD16, also known as low-affinity IgG receptor III (FcγRIII). There are two distinct forms of CD16, CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a 50-65 kDa heterooligomeric polypeptide-anchored transmembrane protein expressed by NK cells, macrophages, and subsets of monocytes1. CD16b is a 48 kDa monomeric glycosylphosphatidylinositol (GPI)-anchored protein expressed on neutrophils1. Upon binding to the Fc portion of IgG or IgG-antigen complex, both CD16 isoforms induce signaling cascades resulting in multiple functions, including phagocytosis, cytokine release, proliferation, degranulation, and antibody dependent cell-mediated cytotoxicity (ADCC)2.

Technical Datasheet:	View
Protein:	CD16
Function:	Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor:	Aggregated IgG, IgG-antigen complex
Research Area:	Cell Biology, Immunology

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